Megazyme® Formate dehydrogenase (Candida boidinii)

300 Units at 25°C; ~ 600 Units at 37°C

SKU No.  700004213  |  Catalog No.  E-FDHCB

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Minimum quantity required: 1
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High purity recombinant Formate dehydrogenase (Candida boidinii) for use in research, biochemical enzyme assays and analytical testing applications.

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Specifications
BrandMegazyme®
EC Number

1.2.1.2 (transferred to EC 1.17.1.9)

Storage Conditions

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions. Check vial for details.

Application ExamplesApplications for the measurement of formate in the food, fermentation, wine, beverage and dairy industries.
FormulationIn 3.2 M ammonium sulphate
Physical FormSuspension
CAS Number

9028-85-7

Concentration~ 75
Synonyms

formate dehydrogenase; formate:NAD+ oxidoreductase

SourceCandida boidinii
Molecular Weight

41331

ExpressionRecombinant from Candida boidinii
Specificity

Catalyses the reaction: Formate + NAD+ = CO2 + NADH

Specific Activity

~ 2 U/mg (25°C, pH 7.6 on formic acid)

Unit Definition

One Unit of formate dehydrogenase is defined as the amount of enzyme required to convert one μmole of formic acid to NADH + CO2 per minute in the presence of NAD+ in potassium phosphate buffer (41 mM), pH 7.6 at 25°C.

Temperature Optima (°C)37
Package Dimensions0.70 in x 0.70 in x 1.60 in
Package Weight0.03 lb