Megazyme® Feruloyl esterase (rumen microorganism)

1,000 Units

SKU No.  700004212  |  Catalog No.  E-FAERU

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High purity recombinant Feruloyl Esterase (rumen microorganism) for use in research, biochemical enzyme assays and analytical testing applications.

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Specifications
BrandMegazyme®
EC Number

3.1.1.73

Storage Conditions

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions. Check vial for details.

Application ExamplesApplications established in biofuels, paper and pulp, food, nutrition, medical and pharmacological industries.
FormulationIn 3.2 M ammonium sulphate
Physical FormSuspension
CAS Number

134712-49-5,
224306-54-1,
224306-55-2

Concentration~ 400
Synonyms

feruloyl esterase; 4-hydroxy-3-methoxycinnamoyl-sugar hydrolase

SourceRumen microorganism
Molecular Weight

29000

ExpressionRecombinant from Rumen microorganism
Specificity

Catalyses the hydrolysis of the 4-hydroxy-3-methoxycinnamoyl (feruloyl) group from an esterified sugar, which is usually arabinose in "natural" substrates.

Specific Activity

~ 30 U/mg (40°C, pH 6.5 on ethyl ferulate)

Unit Definition

One Unit of feruloyl esterase activity is defined as the amount of enzyme required to release one μmole of ferulic acid per minute from ethyl-ferulate (0.39 mM) in sodium phosphate buffer (100 mM), pH 6.5 at 40°C.

Temperature Optima (°C)40
Package Dimensions0.70 in x 0.70 in x 1.60 in
Package Weight0.03 lb