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Enhanced K-Blue® TMB Substrate

SKU No. 308175 | Catalog No.

High activity
Longest shelf-life of Neogen TMB substrates
Low background
Minimum shelf life of 48 months at 2–8°C

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Custom Packaging Available

Enhanced K-Blue® is a one bottle stabilized chromogenic substrate for use with horseradish peroxidase (HRP) immunoassays. It contains both 3,3’,5,5’ tetramethylbenzidine (TMB) and hydrogen peroxide (H₂O₂). Enhanced K-Blue develops a deep blue color in the presence of peroxidase labelled conjugate. Enhanced K-Blue has been optimized for use with microwell applications, especially ELISA. This substrate is not applicable for use with assays requiring a precipitating substrate.

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Specifications
Brand	K-Blue®
Application	Microwell
Intended Use	Research and manufacturing use only
Result Type	Colorimetric
Enzyme	HRP
Sensitivity	High Activity
Storage Conditions	Stable for a minimum of 48 months when stored at 2-8°C.
Wavelength	650 nm or 450 nm with acid stop
Package Weight	0.50 lb

Documents

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Brochure

K-Blue Advanced Brochure - Brochure - English - US Substrates and Reagents Brochure - Brochure - English - US

Insert

Enhanced K-Blue® Substrate (TMB)

Safety Data Sheet (SDS)

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