Amyloglucosidase (Aspergillus niger) Glycerol Free

20 ml

SKU No.  700004184  |  Catalog No.  E-AMGDFNG-20ML

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High purity Amyloglucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
BrandMegazyme
EC Number3.2.1.3
EnzymeAmyloglucosidase
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions

Application ExamplesThis enzyme is recommended for use in Total Dietary Fiber analytical procedures and the Megazyme Total Starch test method. The preparation is free of glycerol so can be used in TDF procedures where glycerol is used as an internal standard (e.g. AOAC Method 2001.03/AACC Method 32-41.01; the Matsutani Method).
FormulationIn buffered solution
Physical FormSolution
CAS Number9032-08-0
CAZy FamilyGH15
Synonymsglucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; glucoamylase
SourceAspergillus niger
Molecular Weight143500
ExpressionFrom Aspergillus niger
SpecificityHydrolysis of terminal (1,4)-linked α-D-glucose residues successively from non-reducing ends of the chains with release of β-D-glucose.
Specific Activity~ 36 U/mg (40°C, pH 4.5 on soluble starch);
~ 400 U/mL on p-nitrophenyl β-maltoside;
~ 6,520 U/mL on soluble starch.
Unit DefinitionOne Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from soluble starch (10 mg/mL) at pH 4.5 at 40°C.
Temperature Optima (°C)70
pH Optima4
Package Dimensions1.10 in x 1.10 in x 2.30 in
Package Weight0.10 lb

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