Megazyme® Amyloglucosidase (Aspergillus niger) Glycerol Free

20 ml

SKU No.  700004184  |  Catalog No.  E-AMGDFNG-20ML

Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

High purity Amyloglucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and analytical testing applications.

View Documents

Megazyme_Scheme-E-AMGDFNG.png
Specifications
BrandMegazyme®
EC Number

3.2.1.3

Storage Conditions

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions

Application ExamplesThis enzyme is recommended for use in Total Dietary Fiber analytical procedures and the Megazyme Total Starch test method. The preparation is free of glycerol so can be used in TDF procedures where glycerol is used as an internal standard (e.g. AOAC Method 2001.03/AACC Method 32-41.01; the Matsutani Method).
FormulationIn buffered solution
Physical FormSolution
CAS Number

9032-08-0

Synonyms

glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; glucoamylase

SourceAspergillus niger
Molecular Weight

143500

ExpressionFrom Aspergillus niger
Specificity

Hydrolysis of terminal (1,4)-linked α-D-glucose residues successively from non-reducing ends of the chains with release of β-D-glucose.

Specific Activity

~ 36 U/mg (40°C, pH 4.5 on soluble starch);

~ 400 U/mL on p-nitrophenyl β-maltoside;

~ 6,520 U/mL on soluble starch.

Unit Definition

One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from soluble starch (10 mg/mL) at pH 4.5 at 40°C.

Temperature Optima (°C)70
Package Dimensions1.10 in x 1.10 in x 2.30 in
Package Weight0.10 lb

Documents

To access certificates, please use our technical document search page.