Veratox® for Fumonisin

SKU No.  700002618  |  Catalog No.  8830

  • Cost effective microwell format ideal for batch testing
  • AOAC-OM approved method
  • Broad range of validated and approved commodities
Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
Veratox® for Fumonisin is a competitive direct ELISA that provides a quantitative analysis of fumonisin in commodities such as barley, corn, DDGS, milo, popcorn, rice, soybeans and wheat.
Specifications
BrandVeratox®
AnalyteFumonisin
PlatformELISA
Result TypeQuantitative
ApprovalsAOAC Official Method 2001.06
Limit of Detection0.2 ppm
Testing Time20.00
Cross ReactivityTotal fumonisins (B1, B2, B3)
Prop 65 Warning
Warning
WARNING: This product can expose you to chemicals including fumonisin B1, which is known to the State of California to cause cancer, and thimerosal and methanol, which are known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
Quantity per Package48 tests
Package Dimensions5.30 in x 4.50 in x 8.10 in
Package Weight2.20 lb
  1. 48 antibody-coated microwells
  2. 48 red-marked mixing wells
  3. 5 yellow-labeled bottles of 0, 1, 2, 4 and 6 ppm fumonisin controls (see Precautions for handling methanol solution)
  4. 1 blue-labeled bottle of fumonisin-HRP conjugate solution
  5. 1 green-labeled bottle of K-Blue® Substrate solution
  6. 1 red-labeled bottle of Red Stop solution
  7. 1 dilution kit that contains 40 dilution bottles prefilled with 7.9 mL of a 10% methanol/water solution (dilution bottles not provided with Neogen item 8831)
  1. Extraction materials (items 4 through 6 available in kit form from Neogen®, item 8052):
    1. 70% methanol solution (Neogen item 8055, 8056) or ACS grade methanol
    2. Distilled or deionized water
    3. 250 mL graduated cylinder (Neogen item 9368)
    4. Container with 125 mL capacity (Neogen item 9428, 9428B)
    5. Neogen filter syringes, Whatman no. 1 filter paper, or equivalent (Neogen item 9420/9430)
    6. Sample collection tubes (Neogen item 9421, 9421B)
  2. High-speed blender (Neogen item 9493, 9477)
  3. Agri-Grind grinder or equivalent (Neogen item 9401/9453)
  4. Scale capable of weighing 2–25 grams ±0.1g (Neogen item 9427)
  5. Microwell reader with a 650 nm filter (Neogen item 9303)
  6. Pipettor, 12-channel (Neogen item 9273)
  7. Pipettor, 100 µL (Neogen item 9272, 9290)
  8. Tips for 100 µL and 12-channel pipettors (Neogen item 9410, 9407, 9417)
  9. Paper towels or equivalent absorbent material
  10. Plastic half gallon bucket for use as waste receptacle
  11. Microwell holder (Neogen item 9402)
  12. Timer (Neogen item 9426)
  13. Waterproof marker
  14. Wash bottle (Neogen item 9400)
  15. 2 reagent boats for 12-channel (Neogen item 9450)
  16. Distilled or deionized water

Technology

Assay Principle

Veratox for Fumonisin allows the user to obtain exact concentrations in parts per million (ppm). Free fumonisin in the samples and controls is allowed to compete with enzyme-labeled fumonisin (conjugate) for the antibody binding sites. After a wash step substrate is added, which reacts with the bound conjugate to produce blue color. More blue color means less fumonisin. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form the standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of fumonisin.

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.

Support

Validation

Our experts are here to help. If you need information beyond what is provided in Validation Reports under the Documents tab, please contact us and we will be happy to assist you. Learn more