Reveal® Q+ MAX for DON

Reveal Q MAX for DON is a single-step lateral flow immunochromatographic assay based on a competitive immunoassay format. The extract is wicked through a reagent zone, which contains antibodies specific for DON conjugated to colloidal gold particles. If DON is present, it will be captured by the particle-antibody complex. The DON-antibody-particle complex then is wicked onto a membrane, which contains a zone of DON conjugated to a protein carrier. This zone captures any uncomplexed DON antibody, allowing the particles to concentrate and form a visible line. As the level of DON in a sample increases, free DON will complex with the antibody-gold particles. This, in turn, allows less antibody-gold to be captured in the test zone. Therefore, as the concentration of DON in the sample increases, the test line density decreases. Algorithms programmed into the reader convert these line densities into a quantitative result displayed in parts per million (ppm). The membrane also contains a control zone where an immune complex present in the reagent zone is captured by an antibody, forming a visible line. The control line will always form regardless of the presence of DON, ensuring the strip is functioning properly.