Yersinia Selective Agar (Schiemann's CIN Agar)
SKU No.  700004572  | Catalog No.  NCM0182A
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Yersinia Selective Agar (Schiemann's CIN Agar)

500 g

SKU No.  700004572  |  Catalog No.  NCM0182A

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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
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Yersinia Selective Agar is used with cefsulodin and novobiocin for the isolation of Yersinia enterocolitica in a laboratory setting. Yersinia Selective Agar is not intended for use in the diagnosis of disease or other conditions in humans.

This medium is based on the work of Schiemann. It is used for the isolation and enumeration of Yersinia spp. from clinical samples and from food. The selective components are sodium desoxycholate, crystal violet, cefsulodin, irgasan and novobiocin. Yersiniae ferment mannitol with an intense, localized, acid production in the center of the colony which produces a red ‘bull’s eye’ appearance. The ratio of transparent border to red centre varies with serotype and environmental strains may appear rough with an irregular edge. Most other enteric bacteria, if they grow, produce a larger colony with a diffuse pinkish center and opaque outer zone.

Specifications
BrandNeogen® Culture Media
AnalyteYersinia spp.
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Peptone Mix22.5 g/L
Mannitol20.0 g/L
Sodium Chloride1.0 g/L
Magnesium Sulphate0.1 g/L
Sodium Pyruvate2.0 g/L
Sodium Deoxycholate0.5 g/L
Neutral red0.03 g/L
Crystal Violet0.001 g/L
Agar12.0 g/L
Grams per Liter58.00
Liters per 500 g9.00
Plates per 500 g470.00
Preparation
  1. Suspend 58 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. DO NOT AUTOCLAVE.
  4. Cool to 45-50ºC and aseptically add 2 vials of C.I.N. Supplement X120.
Previous Item NumberProduct was previously known as Acumedia Product: 7257 Yersinia Selective Agar; and LabM Product: LAB120 Yersinia C.I.N. Agar Base
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb

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Yersinia Selective Agar (Schiemann's CIN Agar)

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