Tryptic Soy Broth, Modified with 20 mg Novobiocin & Acid Digest Casein

500 g

SKU No.  700003360  |  Catalog No.  NCM0137A

Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

TSB, Modified with 20mg Novobiocin & Acid Digest of Casein is used for the selective enrichment of enterohemorrhagic E. coli in foods in a laboratory setting. TSB Modified w/20 mg Novobiocin & Acid Digest of Casein is not intended for use in the diagnosis of disease or other conditions in humans.

The first major outbreak of E. coli O157:H7 was in 1982, and traced to contaminated hamburgers. Other known sources of infection include sprouts, lettuce, salami, unpasteurized milk, juice and/or swimming in or drinking contaminated water. TSB, Modified w/20 mg Novobiocin & Acid Digest of Casein is used to enrich food samples suspected of having low levels of EHEC during pathogen testing. This formulation conforms to the USDA/FSIS Microbiology Laboratory Guidebook.

Specifications
BrandNeogen® Culture Media
AnalyteE. coli, E. coli (EHEC), Enterohemorrhagic
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Enzymatic Digest of Casein17.0 g/L
Acid Digest of Casein10.0 g/L
Sodium Chloride5.0 g/L
Dipotassium Phosphate4.0 g/L
Enzymatic Digest of Soybean Mea3.0 g/L
Dextrose2.5 g/L
Bile Salts No. 31.5 g/L
Novobiocin0.020 g/L
Grams per Liter43.02
Liters per 500 g12.00
Preparation
  1. Dissolve 43.02 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121ºC for 15 minutes.
Previous Item NumberProduct was previously known as Acumedia Product: 7731 Tryptic Soy Broth, Modified with 20 mg/L Novobiocin & Acid Digest of Casein
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb

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