Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar
SKU No.  700003105  | Catalog No.  NCM0052A
Top

Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar

SKU No.  700003105  |  Catalog No.  NCM0052A

Learn more
Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
To order or for more information contact us
To order or for more information contact us

Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar is used for the selective isolation of Vibrio cholerae and other enteropathogenic vibrios according to ISO 21872-1:2017 and ISO 21872-2:2007. Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar is not intended for use in the diagnosis of disease or other conditions in humans.

TCBS Agar is also called Vibrio Selective Agar, and prepared according to the formula of Kobayashi et al. This formula is a modification of the selective medium from Nakanishi. All pathogenic Vibrio spp., except Vibrio hollisae, will grow on TCBS Agar. This highly selective agar meets the nutritional requirements of Vibrio spp. Vibrio spp. are able to grow in media containing increased salt concentrations, and some species are halophilic. Infections have been associated with ingestion of contaminated water and consumption of contaminated shellfish or seafood. Vibrio spp. are natural inhabitants of seawater.

According to ISO 21872-2, pre-incubation in Alkaline Saline Peptone Water (ASPW) (NCM0175) is required.

Specifications
BrandNeogen® Culture Media
AnalyteVibrio spp.
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Yeast Extract5.0 g/L
Enzymatic Digest of Casein5.0 g/L
Enzymatic Digest of Animal Tissue5.0 g/L
Sodium Citrate10.0 g/L
Sodium Thiosulfate10.0 g/L
Bile Salts8.0 g/L
Sucrose20.0 g/L
Sodium Chloride10.0 g/L
Ferric Citrate1.0 g/L
Bromothymol Blue0.04 g/L
Thymol Blue0.04 g/L
Agar*14.0 g/L
Grams per Liter88.00
Liters per 500 g6.00
Preparation
  1. Suspend 88 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. DO NOT AUTOCLAVE.
Previous Item NumberProduct was previously known as Acumedia Product: 7210 TCBS Agar; and LabM Product: LAB096 T.C.B.S. Cholera Medium
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb

Support

Get in Touch

Validation

Our experts are here to help. If you need information beyond what is provided in Validation Reports under the Documents tab, please contact us and we will be happy to assist you. Learn more