Oxford Listeria Agar
SKU No.  700003121  | Catalog No.  NCM0056A
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Oxford Listeria Agar

SKU No.  700003121  |  Catalog No.  NCM0056A

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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
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Oxford Listeria Agar Base is used with antimicrobics for the selective isolation of Listeria spp. in a laboratory setting. Oxford Listeria Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in food industries. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission included turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage and river water. Oxford Listeria Agar is prepared according to the formulation of Curtis et al. Listeria spp. grow over a pH range of 5.0 - 9.6 and survive in food products with pH levels outside these parameters.
Specifications
BrandNeogen® Culture Media
AnalyteListeria monocytogenes
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Columbia Blood Agar Base39.0 g/L
Esculin1.0 g/L
Ferric Ammonium Citrate0.5 g/L
Lithium Chloride15.0 g/L
Agar2.0 g/L
Grams per Liter57.50
Liters per 500 g9.00
Plates per 500 g470.00
Preparation
  1. Suspend 57.5 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45 - 50°C.
  5. Aseptically add 2 vials of NCM4047-0.5* Oxford Supplement (with Natamycin), each reconstituted using 5 mL sterile 50% ethanol, or NCM4070-0.5* Oxford Supplement (with Cycloheximide), each reconstituted using 5 mL sterile 50% ethanol and mix well before dispensing.
  • Modified Oxford Listeria Agar: Perform steps 1-4 above and aseptically add 2 vials of NCM4080-0.5*
  • Modified Oxford Agar Supplement: each reconstituted using 5mL sterile deionized/RO water.

*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.

Previous Item NumberProduct was previously known as LabM Products: LAB122 Listeria Isolation Medium (Oxford) & LAB206 Listeria Isolation Media
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb

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