Nutrient Broth No. 2

Preparation for use as Nutrient Broth

1. Dissolve 25 grams of the medium in one liter of purified water.
2. Heat with frequent agitation to completely dissolve the medium if necessary.
3. Autoclave at 121°C for 15 minutes. Mix well.

Preparation for use as Preston Broth

1. Dissolve 25 grams of the medium in one liter of purified water.
2. Heat with frequent agitation to completely dissolve the medium if necessary.
3. Autoclave at 121°C for 15 minutes.
4. When using Nutrient Broth No. 2 to prepare Preston Broth add 2 vials of NCM4038-0.5* each reconstituted using 5mL sterile 50% ethanol, 2 vials of NCM4033-0.5*, each reconstituted using 5 mL sterile deionized/RO water and 50 mL of lysed blood. Mix well.

*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.