MacConkey Broth

SKU No.  700003133  |  Catalog No.  NCM0060A

Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
MacConkey Broth is used for the detection of coliform bacteria in milk and water. Conforms to Harmonized USP/EP/JP Requirements and is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for the selective enrichment of Escherichia coli from non-sterile samples. Conforms to Harmonized USP/EP/JP performance specification. Gelatin peptone provides a source of nitrogen, while lactose is a fermentable carbohydrate. Ox bile acts a selective agent inhibiting most Gram-positive organisms and bromocresol purple acts as a pH indicator. A color change from purple to yellow indicates growth of a bile tolerant, lactose fermenting organism such as Escherichia coli. According to the Harmonized USP/EP/JP, MacConkey Broth is used as a selective enrichment broth, with subculture performed onto MacConkey Agar.
Specifications
BrandNeogen® Culture Media
AnalyteE. coli
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Pancreatic Digest of Gelatin20.0 g/L
Lactose Monohydrate10.0 g/L
Dehydrated Ox Bile5.0 g/L
Bromocresol Purple0.01 g/L
Grams per Liter35.00
Liters per 500 g10.00
Preparation
  1. Dissolve 35 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Dispense into tubes containing Durham tubes.
  4. Autoclave at 121°C for 15 minutes.
Previous Item NumberProduct was previously known as Acumedia Product: 7185 MacConkey Broth; and LabM Product: HP005 MacConkey Broth (USP/EP/JP)
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb