m-TEC Agar
SKU No.  700003672  | Catalog No.  NCM0291A
Top

m-TEC Agar

500 g

SKU No.  700003672  |  Catalog No.  NCM0291A

Learn more
Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
To order or for more information contact us.
To order or for more information contact us.

Escherichia coli (E. coli) is used as an indicator of fecal pollution in water. Several tests are available for enumerating E. coli based on its ability to grow at elevated temperatures and indole production. The membrane filter procedure is recognized in Standard Methods as an alternate test procedure. m-TEC is an abbreviation for membrane thermotolerant E. coli.

In 1981, Dufour et al. developed a simple and accurate membrane filter technique for rapid enumeration of E. coli. In this study, the researchers were able to quantitate E. coli on m-TEC Agar within 24 hours without requiring subculture and identification of isolates. Dufour et al. recovered E. coli from marine, estuarine, and freshwater samples.

Specifications
BrandNeogen® Culture Media
PlatformDehydrated Culture Media
Formula
FormulaLiter
Enzymatic Digest of Animal Tissue5.0 g/L
Yeast Extract3.0 g/L
Lactose10.0 g/L
Sodium Chloride7.5 g/L
Potassium Phosphate4.3 g/L
Sodium Lauryl Sulfate0.2 g/L
Sodium Deoxycholate0.1 g/L
Bromocresol Purple0.08 g/L
Bromophenol Red0.08 g/L
Agar15.0 g/L
Grams per Liter45.30
Liters per 500 g11.00
Plates per 500 g2,200.00
Preparation
  1. Suspend 45.3 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Dispense 4 – 5 mL amounts into 10 x 50 mm petri dishes, allow to solidify.

Urea Substrate

  1. Combine 2 g urea and 10 mg phenol red in 100 mL purified water.
  2. Adjust pH to 5.0 ± 0.3
  3. Store at 2 - 8°C. Use within one week.

Note: Other methods may recommend an alternative pH. Prepare substrate according to recommended guidelines.

Previous Item NumberProduct was previously known as Acumedia product: 7421 m-TEC Agar
Package Dimensions3.60 in x 3.60 in x 6.50 in
Package Weight0.50 lb