m-HPC Agar

SKU No.  700003664  |  Catalog No.  NCM0284A

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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

m-HPC Agar is used for the enumeration of heterotrophic organisms in water by the membrane filtration technique in a laboratory setting. m-HPC Agar is not intended for use in the diagnosis of disease or other conditions in humans.

m-HPC Agar, also known as m-Heterotrophic Plate Count Agar, was developed by Taylor and Geldreich in 1979. Monitoring the levels of heterotrophic bacteria, those microorganisms that can grow on minimal organic nutrients, is currently performed to indicate problems with treatment methods within distribution systems. There is evidence that heterotrophic bacteria are not hazardous to healthy individuals, but may be opportunistic pathogens capable of infecting individuals with impaired immune systems.

m-HPC Agar is used in standard methods for the detection of heterotrophic bacteria using the membrane filtration technique. The membrane filter technique is a microbiological method used in testing large volume samples. The concentration of larger samples on a membrane filter is a key benefit of the membrane filtration procedure. Several studies reported m-HPC Agar as a suitable alternate medium for standard total plate count agar.

Specifications
BrandNeogen® Culture Media
AnalyteHeterotrophic
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Peptone20.0 g/L
Gelatin25.0 g/L
Agar15.0 g/L
Grams per Liter60.00
Liters per 500 g8.00
Plates per 500 g450.00
Preparation
  1. Suspend 60 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Add 10 mL of Glycerol to 1 Liter of solution.
  4. Autoclave at 121°C for 5 minutes.
  5. Cool to 45-50°C.
Previous Item NumberProduct was previously known as Acumedia Product: 7690 m-HPC Agar
Package Dimensions10.20 in x 10.20 in x 11.90 in
Package Weight1.20 lb