m-Endo Agar
SKU No.  700003324  | Catalog No.  NCM0123A
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m-Endo Agar

500 g

SKU No.  700003324  |  Catalog No.  NCM0123A

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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
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m-Endo Agar is used for the enumeration of coliforms in water by the membrane filtration method in a laboratory setting. m-Endo Agar is not intended for use in the diagnosis of disease or other conditions in humans.

The coliform group, especially Escherichia coli, are used as indicators of fecal pollution in water, for assessing the effectiveness of water treatment and disinfection, and for monitoring water quality. m-Endo Agar is prepared according to the formula of McCarthy, Delaney, and Grasso, and used in testing water for coliforms by a two-step membrane filtration procedure. Lauryl Tryptose Broth is used as the preliminary enrichment, resulting in higher coliform counts.

The American Public Health Association (APHA) recommends m-Endo Agar in standard total coliform membrane filtration procedure for testing water, wastewater, and foods. The US EPA specifies using m-Endo Agar in the total coliform methods for testing water. m-Endo Agar is also known as LES (Lawrence Experimental Station) Endo Agar.

Specifications
BrandNeogen® Culture Media
AnalyteColiforms
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Lactose9.4 g/L
Tryptose7.5 g/L
Enzymatic Digest of Casein3.7 g/L
Enzymatic Digest of Animal Tissue3.7 g/L
Sodium Chloride3.7 g/L
Potassium Phosphate, Dibasic3.3 g/L
Sodium Sulfite1.6 g/L
Yeast Extract1.2 g/L
Potassium Phosphate, Monobasic1.0 g/L
Basic Fuchsin0.8 g/L
Sodium Deoxycholate0.1 g/L
Sodium Lauryl Sulfate0.05 g/L
Agar15.0 g/L
Grams per Liter51.00
Liters per 500 g12.00
Plates per 500 g670.00
Preparation
  1. Suspend 51 g of the medium in 1 liter of purified water containing 20 mL of non-denatured Ethanol.
  2. Heat with frequent agitation and boil to completely dissolve the medium.
  3. Avoid overheating. DO NOT AUTOCLAVE.
Previous Item NumberProduct was previously known as Acumedia Product: 7724 m-Endo Agar; and LabM Product: LAB060 m-Endo Agar
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb