HC Agar Base
SKU No.  700003416  | Catalog No.  NCM0155A
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HC Agar Base

500 g

SKU No.  700003416  |  Catalog No.  NCM0155A

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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
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HC Agar Base is used with Tween 80 (Polysorbate 80) for the enumeration of molds in cosmetics in a laboratory setting. HC Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

Methods for isolating molds from cosmetic products require incubation for 5 - 7 days using traditional agar media. In 1986, Mead and O’Neill described a new medium, HC Agar, for enumerating molds in cosmetic products. The formulation of HC Agar decreased incubation time to 3 days at 27.5 ± 0.5°C for molds. HC Agar Base, based on the HC Agar formula of Mead and O’Neill, is supplemented with Tween 80 (Polysorbate 80) to prepare HC Agar.

Specifications
BrandNeogen® Culture Media
AnalyteMolds
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Enzymatic Digest of Casein2.5 g/L
Enzymatic Digest of Animal Tissue2.5 g/L
Yeast Extract5.0 g/L
Dextrose20.0 g/L
Disodium Phosphate3.5 g/L
Monopotassium Phosphate3.4 g/L
Ammonium Chloride1.4 g/L
Sodium Carbonate1.0 g/L
Magnesium Sulfate0.06 g/L
Chloramphenicol0.1 g/L
Agar15.0 g/L
Grams per Liter54.50
Liters per 500 g9.00
Plates per 500 g500.00
Preparation
  1. Suspend 54.5 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Add 20 mL of Tween 80/Polysorbate 80 (7992) and mix.
  4. Autoclave at 121°C for 15 minutes.
  5. Cool to 45-50°C.
Previous Item NumberProduct was previously known as Acumedia Product: 7520 HC Agar Base
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb