Harlequin® Modified Chromogenic Agar for Salmonella Esterase(mCASE)

mCASE utilizes a dual chromogenic system to differentiate between Salmonella and non-target organisms that grow on the agar. The first chromogen is a target for esterase activity present in all Salmonella species, and results in blue/green colonies. The second chromogen is a target for β-glucosidase activity, present in non-target organisms that are able to grow on the agar and also possess esterase activity. Utilization of the second chromogen masks the presentation of the first, resulting in black colonies in non-Salmonella organisms. All other organisms are either inhibited or grow colorless on the agar. The medium contains an opacity agent that provides a white background, facilitating easy reading and identification. The formulation is the result of modification of CASE (NCM1006), allowing for improved recovery and detection of S. Typhi and S. Paratyphi. The media is able to detect non-motile Salmonella as well as monophasic variants. The media is also able to detect serovars that traditionally present weak esterase activity), and lactose positive strains. Sub-culture should be performed from a selective enrichment.

mCASE is not intended for use with food testing, instead CASE (NCM1006) should be used.