Glucose OF Medium
SKU No.  700004539  | Catalog No.  NCM0152A
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Glucose OF Medium

SKU No.  700004539  |  Catalog No.  NCM0152A

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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
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Glucose OF Medium is used for the confirmation of Enterobacteriaceae in foods according to ISO 21528-1&2:2017, and is not intended for use in the diagnosis of disease or other conditions in humans.

Enzymatic digest of casein provides nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Dipotassium phosphate acts as a buffer system. Glucose is the fermentable carbohydrate providing carbon and energy. Bromothymol blue is the pH indicator.

Colonies of presumptive Enterobacteriaceae should be confirmed by means of tests for the fermentation of glucose and the presence of oxidase. If the colonies are oxidase-negative and glucose-positive, the sample shall be regarded as being positive for Enterobacteriaceae. ISO 21528 :2017 recommends this medium to confirm the presumptive colonies of Enterobacteriaceae by fermentation test.

Specifications
BrandNeogen® Culture Media
AnalyteEnterobacteriaceae
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Enzymatic Digest of Casein2.0 g/L
Dipotassium Hydrogen Phosphate0.3 g/L
Glucose10.0 g/L
Sodium Chloride5.0 g/L
Bromthymol Blue0.08 g/L
Agar4.0 g/L
Grams per Liter21.40
Liters per 500 g23.00
Plates per 500 g1,280.00
Preparation
  1. Dissolve 21.4 grams of the medium into one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium if necessary.
  3. Autoclave at 121ºC for 15 minutes.
  4. Cool to 45-50ºC.
Previous Item NumberProduct was previously known as LabM Product: LAB227
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb

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