Neogen® Fraser Broth
SKU No.  700003097  | Catalog No.  NCM0050A
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Neogen® Fraser Broth

SKU No.  700003097  |  Catalog No.  NCM0050A

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Fraser Broth is a selective medium used for the secondary enrichment of Listeria monocytogenes and Listeria spp., as described in ISO 11290-1:2017. Fraser Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Fraser Broth was developed as a modification of UVM medium (NCM0012) and is based on the formula as described by Fraser and Sperber. Listeria spp. hydrolyze the esculin to esculetin which reacts with the ferric ammonium citrate (X211 or 7984) resulting in a black precipitate and a visible positive reaction.

Specifications
BrandNeogen® Culture Media
AnalyteListeria spp.
PlatformDehydrated Culture Media
Mass500 g
Grams per Liter55.00
Liters per 500 g9.00
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb

Additional Information

Ferric Ammonium Citrate Supplement (X211) or Fraser broth base supplement (7984) may be used

Documents

To access certificates, please use our technical document search page.

Neogen® Fraser Broth Product Instructions - English - US
Fraser Broth - English - US Fraser Broth - German - Belgium Fraser Broth - French - France/Monaco Fraser Broth - English - Ireland Fraser Broth - Bulgarian - Bulgaria Fraser Broth - Korean - Korea Fraser Broth - Finnish - Finland Fraser Broth - French - Luxembourg Fraser Broth - Romanian - Romania Fraser Broth - Italian - Italy Fraser Broth - English - Canada Fraser Broth - Icelandic - Iceland Fraser Broth - Slovak - Slovakia Fraser Broth - Hungarian - Hungary Fraser Broth - Estonian - Estonia Fraser Broth - Spanish - US Fraser Broth - Norwegian - Norway Fraser Broth - French - Switzerland Fraser Broth - Dutch - Netherlands Fraser Broth - Danish - Denmark Fraser Broth - Dutch - Belgium Fraser Broth - French - Canada Fraser Broth - English - Great Britain Fraser Broth - French - Belgium Fraser Broth - Croatian - Croatia Fraser Broth - Polish - Poland Fraser Broth - English - Malta Fraser Broth - Greek - Greece Fraser Broth - Portuguese - Portugal Fraser Broth - Italian - Switzerland Fraser Broth - Spanish - Spain Fraser Broth - German - Luxembourg Fraser Broth - Latvian - Latvia Fraser Broth - Swedish - Sweden Fraser Broth - Slovene - Slovenia Fraser Broth - German - Austria Fraser Broth - Lithuanian - Lithuania Fraser Broth - Maltese - Malta Fraser Broth - Czech - Czech Republic Fraser Broth - Greek - Cyprus Fraser Broth - German - Switzerland Fraser Broth - German - Germany/Liechtenstein

Technology

Lithium chloride suppresses the growth of Enterococci in the medium, as Enterococci can also hydrolyze esculin also. Enzymatic digest of animal tissues, casein, meat extract and yeast extract provide the essential vitamins, minerals, amino acids, nitrogen and carbon, while sodium chloride maintains the osmotic balance. The selective components acriflavine and nalidixic acid are blended into the base powder and inhibit the growth of Escherichia coli. Ferric ammonium citrate is added to the tempered broth after sterilization. According to ISO 11290-1:2017, the test portion is inoculated into the primary enrichment medium Half Fraser (Demi-Fraser) Broth (ISO), followed by secondary enrichment in Fraser Broth (ISO) and then sub-cultured onto Harlequin Listeria Chromogenic Agar according to Ottaviani & Agosti (NCM1004). The medium conforms to the performance and formulation requirements of ISO 11290-1:2017.