DC Medium with BCIG
SKU No.  700003284  | Catalog No.  NCM0112A
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DC Medium with BCIG

SKU No.  700003284  |  Catalog No.  NCM0112A

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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
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DC Medium with BCIG is used in the chromogenic differentiation of E. coli from other coliforms in water samples using the membrane filtration method in a laboratory setting. DC Medium with BCIG is not intended for use in the diagnosis of disease or other conditions in humans.

DC (Differential Coliform) Medium with BCIG is a selective and differential medium for the presumptive identification of E. coli. This medium is enhanced by the addition of a chromogenic agent, BCIG, 5-bromo-4-chloro-3-indolyl-ß-D-glucuronide to detect glucuronidase activity. The presence of the enzyme ß-D-glucuronidase differentiates most E. coli spp. from other coliforms, and is the same enzyme used in the MUG reaction. BCIG reacts slightly differently, and when released into the medium is insoluble, accumulating within the cell, imparting a blue to purple color to presumptive E. coli colonies.

Specifications
BrandNeogen® Culture Media
AnalyteColiforms, E. coli
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Lactose10.0 g/L
Tryptose10.0 g/L
Yeast Extract3.0 g/L
Sodium Chloride5.0 g/L
Proteose Peptone5.0 g/L
Bile Salts1.5 g/L
BCIG0.2 g/L
Neutral Red0.08 g/L
Agar15.0 g/L
Grams per Liter49.80
Liters per 500 g10.00
Plates per 500 g550.00
Preparation
  1. Suspend 49.8 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. DO NOT AUTOCLAVE.
  4. Cool to 45 - 50°C.
  5. Aseptically add 1.2 mL of a sterile 10 mg/mL solution of Cefsulodin and mix well.
Previous Item NumberProduct was previously known as Acumedia Product: 7704 DC Medium with BCIG
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb