Cystine Lactose Electrolyte Deficient (CLED) Agar
SKU No.  700003061  | Catalog No.  NCM0034A
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Cystine Lactose Electrolyte Deficient (CLED) Agar

SKU No.  700003061  |  Catalog No.  NCM0034A

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Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
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Cystine Lactose Electrolyte Deficient (CLED) Agar is used for the differentiation and enumeration of microorganisms in urine. A medium for urine culture where the absence of electrolytes inhibits the swarming of Proteus spp. Cystine is added for the benefit of those organisms which have a specific cystine requirement. Differentiation of lactose and non-lactose fermenters is achieved using bromothymol blue as pH indicator. This medium supports the growth of Streptococcus pyogenes and most other fastidious organisms that do not require blood.
Specifications
BrandNeogen® Culture Media
AnalyteColiforms, E. coli, Enterobacteriaceae, Enterococci, Pseudomonas, Staphylococcus, Streptococci
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Balanced Peptone4.0 g/L
Beef Extract3.0 g/L
Tryptone4.0 g/L
Lactose10.0 g/L
L-Cystine0.128 g/L
Bromothymol Blue0.02 g/L
Agar15.0 g/L
Grams per Liter36.00
Liters per 500 g14.00
Plates per 500 g760.00
Preparation
  1. Suspend 36 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.
Previous Item NumberProduct was previously known as Acumedia Product: 7122 CLED Agar; and LabM Product: LAB041 CLED Medium (Mackey and Sandys)
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb

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