Columbia Broth

SKU No.  700003440  |  Catalog No.  NCM0162A

Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

Columbia Broth is used for the cultivation of a wide variety of fastidious microorganisms in a laboratory setting. Columbia Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Columbia Broth is prepared according to the formula described by Morello and Ellner. During their study, Columbia Broth was developed as a medium for blood cultures and found superior to a commonly used broth for growth of Staphylococcus aureus, E. coli, and streptococci.

Specifications
BrandNeogen® Culture Media
AnalyteNon-selective
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Enzymatic Digest of Casein5.0 g/L
Enzymatic Digest of Animal Tissue5.0 g/L
Yeast Extract10.0 g/L
Enzymatic Digest of Heart Muscle3.0 g/L
Sodium Chloride5.0 g/L
Dextrose2.5 g/L
L-Cystine0.1 g/L
Magnesium Sulfate0.1 g/L
Ferrous Sulfate0.02 g/L
Tris (hydroxymethyl) aminomethane0.83 g/L
Tris (hydroxymethyl) aminomethane-HCl2.86 g/L
Sodium Carbonate0.6 g/L
Grams per Liter35.00
Liters per 500 g14.00
Plates per 500 g780.00
Preparation
  1. Dissolve 35 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.
Previous Item NumberProduct was previously known as Acumedia Product: 7127 Columbia Broth
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb