Campy Blood-Free Selective Medium (Modified CCDA)
SKU No.  700003069  | Catalog No.  NCM0042A
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Campy Blood-Free Selective Medium (Modified CCDA)

SKU No.  700003069  |  Catalog No.  NCM0042A

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Campy Blood-Free Selective Medium (CCDA) is used with cefoperazone for the selective isolation of Campylobacter spp. in a laboratory setting. Campy Blood-Free Selective Medium (CCDA) is not intended for use in the diagnosis of disease or other conditions in humans

Campy Blood-Free Selective Medium (CCDA) was described by Bolton et al. This medium was formulated to replace blood with charcoal, ferrous sulfate, and sodium pyruvate. To improve selectivity, cefoperazone replaced cephazolin in the original formulation. Bolton et al recommended incubating inoculated plates at 37°C to improve isolation rates. Yeast and fungal contaminants are inhibited with the addition of amphotericin B. Campy Blood-

Free Selective Medium (CCDA) is recommended for food testing.

Specifications
BrandNeogen® Culture Media
AnalyteCampylobacter spp.
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Nutrient Broth No. 225.0 g/L
Charcoal4.0 g/L
Casein Acid Hydrolysate3.0 g/L
Sodium Deoxycholate1.0 g/L
Ferrous Sulfate0.25 g/L
Sodium Pyruvate0.25 g/L
Agar12.0 g/L
Grams per Liter45.50
Liters per 500 g11.00
Plates per 500 g600.00
Preparation
  1. Suspend 45.5 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool medium to 45 - 50°C and aseptically add 4 mL of a filtered sterilized aqueous solution containing 32 mg of cefoperazone.
  5. Mix well and pour into petri dishes.
Previous Item NumberProduct was previously known as Acumedia Product: 7527 Campy-Blood Free Selective Medium (CCDA)
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb

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