Blood Agar Base No. 2
SKU No.  700004423  | Catalog No.  NCM0040A
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Blood Agar Base No. 2

SKU No.  700004423  |  Catalog No.  NCM0040A

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Blood Agar Base No. 2 is used with blood for the isolation and cultivation of a wide variety of fastidious microorganisms. Blood Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. A very rich agar base which, with the addition of blood, is capable of growing fastidious organisms. The medium gives colonial appearances, hemolysis patterns and pigment production of indicative value. When the blood is 'chocolated' the medium gives good recovery of Haemophilus spp. The medium can be made selective for various groups by the addition of appropriate antibiotic mixtures eg: Streptococcus spp. Colistin/Oxolinic acid (X013) Gardnerella spp. Colistin/Oxolinic acid (X011) C. perfringens Neomycin (X015) (X016) Staphylococcus/Streptococcus spp. Colistin/Naladixic acid (X012)
Specifications
BrandNeogen® Culture Media
AnalyteAnaerobes, Brucella, Campylobacter spp., Clostridium, Gardnerella, Haemophilus, Helicobacter, Listeria spp., Neisseria, Staphylococci, Streptococci
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Tryptose15.0 g/L
Soy Peptone2.5 g/L
Yeast Extract5.0 g/L
Sodium Chloride5.0 g/L
Agar12.0 g/L
Grams per Liter39.50
Liters per 500 g13.00
Plates per 500 g690.00
Preparation
  1. Suspend 39.5 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C .
  5. Aseptically add 5 – 7% sterile, defibrinated horse or sheep blood.
  6. Mix well before pouring.
Previous Item NumberProduct was previously known as Acumedia Product: 7266 Blood Agar Base No. 2; and LabM Product: LAB015 Blood Agar Base No. 2
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb