APT Agar
SKU No.  700003432  | Catalog No.  NCM0159A
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APT Agar

SKU No.  700003432  |  Catalog No.  NCM0159A

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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
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APT Agar is used for the cultivation of heterofermentative lactobacilli in a laboratory setting. APT Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Evans and Niven investigated the cultivation of heterofermentative lactobacilli, causing the faded or green discoloration of cured meat products. Deibel, Evans, and Niven tested thiamine-requiring bacteria, specifically Lactobacillus viridescens. Their formulations led to the development of APT Agar.

Lactic acid bacteria, a group of acid-producing bacteria, include the genera Streptococcus, Leuconostoc, Pediococcus, and Lactobacillus. These organisms are widespread in nature, associated with bacterial spoilage of foods including dairy products, meat, and vegetables. APT Agar is used for cultivating heterofermentative lactic acid bacteria from food products.

Specifications
BrandNeogen® Culture Media
AnalyteHeterofermentative lactobacilli
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Enzymatic Digest of Casein10.0 g/L
Yeast Extract7.5 g/L
Sodium Chloride5.0 g/L
Potassium Phosphate5.0 g/L
Sodium Citrate5.0 g/L
Dextrose10.0 g/L
Polysorbate 800.2 g/L
Magnesium Sulfate0.8 g/L
Manganese Chloride0.14 g/L
Ferrous Sulfate0.04 g/L
Sodium Carbonate1.25 g/L
Agar13.5 g/L
Grams per Liter58.00
Liters per 500 g9.00
Plates per 500 g470.00
Preparation
  1. Suspend 58 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 118 - 121°C for 15 minutes.
  4. Cool to 45-50°C.
Previous Item NumberProduct was previously known as Acumedia Product: 7302 APT Agar
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb

Documents

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APT Agar - Technical Specifications Sheet - English - US

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