Megazyme® Phosphate Assay Kit
100 assays (manual) / 400 assays (auto-analyser)
SKU No. 700004326 | Catalog No. K-PHOS
- Chemically defined detection method
- All reagents stable for > 2 years after preparation
- Rapid reaction (~ 20 min)
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Suitable for manual and auto-analyser formats
The Phosphate Assay Kit is a rapid, simple, reliable and accurate method for the specific measurement and analysis of phosphate in water, foodstuffs, beverages and other materials. The phosphate detection reaction employs the defined chemical substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG). In the presence of phosphate, the substrate is converted enzymatically by purine nucleoside phosphorylase (PNPase) to the products ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine. An application note describes the dephosphorylation reaction of phytic acid using phytase and alkaline phosphatase (sold separately), and subsequent phosphate detection using the reagents provided in K-PHOS.
This method is suitable for analysis using spectrophotometer and auto-analyser.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.
| Specifications | ||
|---|---|---|
| Brand | Megazyme® | |
| Analyte | Phosphate | |
| Platform | Spectrophotometer, Auto-analyser | |
| Result Type | UV Absorbance | |
| Limit of Detection | 0.16 mg/L | |
| Storage Conditions | Storage Temperature: Short term stability: 2-8°C, Long term stability: See individual component labels Stability: > 2 years under recommended storage conditions | |
| Wavelength | 360 nm | |
| Application Examples | Processed foods and drinks, bakery products, dairy products, waste water samples, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, etc.). | |
| Signal Response | Increase | |
| Linear Range | 0.1 to 10 μg of phosphate per assay | |
| Reaction Time | ~ 20 min | |
| Package Dimensions | 10.00 in x 5.00 in x 2.00 in | |
| Package Weight | 0.47 lb | |
Documents
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