Megazyme® Phosphotransacetylase (Bacillus subtilis)

3,000 Units

SKU No.  700004235  |  Catalog No.  E-PTABS

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Minimum quantity required: 1
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High purity recombinant phosphotransacetylase (Bacillus subtilis) for use in research, biochemical enzyme assays and analytical testing applications.

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Specifications
BrandMegazyme®
EC Number

2.3.1.8

Storage Conditions

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions. Check vial for details.

Application ExamplesApplications for the measurement of acetic acid in the food, fermentation, wine, beverage and dairy industries.
FormulationIn 3.2 M ammonium sulphate
Physical FormSuspension
CAS Number

9029-91-8

Concentration~ 3000 U/mL
Synonyms

phosphate acetyltransferase; acetyl-CoA:phosphate acetyltransferase

SourceBacillus subtilis
Molecular Weight

36953

ExpressionRecombinant from Bacillus subtilis
Specificity

Catalyses the reaction: Coenzyme A + acetyl phosphate = acetyl-CoA + phosphate

Specific Activity

~ 3,500 U/mg (25°C, pH 7.4 on Coenzyme A)

Unit Definition

One Unit of phosphotransacetylase activity is defined as the amount of enzyme required to release one µmole of acetyl-CoA per minute from Coenzyme A (0.44 mM) in Tris.HCl buffer (86 mM) pH 7.4 at 25°C.

Temperature Optima (°C)25
Package Dimensions0.70 in x 0.70 in x 1.60 in
Package Weight0.04 lb