Megazyme® Pectate Lyase (Aspergillus sp.)

7,000 Units in 50% aqueous glycerol

SKU No.  700004231  |  Catalog No.  E-PCLYAN

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Minimum quantity required: 1
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High purity Pectate lyase (Aspergillus sp.) for use in research, biochemical enzyme assays and analytical testing applications.

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Specifications
BrandMegazyme®
EC Number

4.2.2.2

Storage Conditions

Storage Temperature: Below -10°C

Stability: > 1 year under recommended storage conditions

Application ExamplesSuitable for pectin identification according to USP method [Pectin 9000-69-5]; Pectin Identification Procedure. Applications in carbohydrate and biofuels research.
FormulationIn 50% (v/v) glycerol
Physical FormSolution
CAS Number

9015-75-2

Concentration~ 1400
Synonyms

pectate lyase; (1→4)-alpha-D-galacturonan lyase

SourceAspergillus sp.
Molecular Weight

45000

ExpressionPurified from Aspergillus sp.
Specificity

Eliminative cleavage of (1,4)-α-D-galacturonan to give oligosaccharides with 4-deoxy-α-D-galact-4-enuronosyl groups at their non-reducing ends

Specific Activity

~ 200 U/mg (40°C, pH 8.0 on polygalacturonic acid)

Unit Definition

One Unit of pectate lyase activity is defined as the amount of enzyme required to release one μmole of galacturonic acid from polygalacturonic acid (2.5 mg/mL) per min in Tris.HCl buffer (50 mM), pH 8.0 at 40°C.

Temperature Optima (°C)55
Package Dimensions0.80 in x 0.80 in x 1.90 in
Package Weight0.04 lb