Megazyme® Hexokinase/Glucose-6-phosphate dehydrogenase

10 ml Hexokinase (420 U/ml) + G6P-DH (210 U/ml)

SKU No.  700004221  |  Catalog No.  E-HKGDH

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Minimum quantity required: 1
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High purity Hexokinase (yeast) / Glucose-6-phosphate dehydrogenase (G6P-DH) (Leuconostoc mesenteroides) for use in research, biochemical enzyme assays and analytical testing applications.

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Specifications
BrandMegazyme®
EC Number

Hexokinase: 2.7.1.1
G6P-DH: 1.1.1.49

Storage Conditions

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions

Application ExamplesApplications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.
FormulationIn 3.2 M ammonium sulphate
Physical FormSuspension
CAS Number

Hexokinase: 9001-51-8
G6P-DH: 9001-40-5

ConcentrationHexokinase: ~ 420 U/mL
G6P-DH: ~ 210 U/mL
Synonyms

Hexokinase: Hexokinase; ATP:D-hexose 6-phosphotransferase
G6P-DH: glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase

SourceYeast
ExpressionHexokinase: Recombinant from yeast
G6P-DH: Recombinant from Leuconostoc mesenteroides
Specificity

Hexokinase: Catalyses the reaction: ATP + D-hexose = ADP + D-hexose 6-phosphate Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine.

Glucose-6-phosphate dehydrogenase: Catalyses the reaction: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+

Unit Definition

Hexokinase: One Unit of hexokinase activity is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.5 and 25°C.
G6P-DH: One Unit of Glucose-6-phosphate dehydrogenase activity is the amount of enzyme required to convert one μmole of glucose-6-phosphate to 6-phosphogluconate per minute, in the presence of NADP+ at pH 7.8 and 25°C.

Temperature Optima (°C)40
Package Dimensions0.80 in x 0.80 in x 1.90 in
Package Weight0.06 lb