Megazyme® α-L-Arabinofuranosidase (Aspergillus niger)

480 Units on pNP-α-Arabf at 40oC

SKU No.  700004179  |  Catalog No.  E-AFASE

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High purity α-L-Arabinofuranosidase (Aspergillus niger) for use in research, biochemical enzyme assays and analytical testing applications.

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Specifications
BrandMegazyme®
EC Number

3.2.1.55

Enzymeα-Arabinofuranosidase
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions

Application ExamplesApplications in carbohydrate and biofuels research.
FormulationIn 3.2 M ammonium sulphate
Physical FormSuspension
CAS Number

9067-74-7

CAZy FamilyGH51
Concentration~ 300
Synonyms

non-reducing end alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase

SourceAspergillus niger
Molecular Weight

62000

ExpressionFrom Aspergillus niger
Specificity

Hydrolysis of α-1,2- and α-1,3-linked L-arabinofuranose residues from arabinoxylans and branched arabinans. Hydrolyses α-1,5-linked arabino-oligosaccharides at a much lower rate.

Specific Activity

~ 32 U/mg (40°C, pH 4.0 on p-nitrophenyl-α-L-arabinofuranoside);
~ 12.6 U/mg (on sugar-beet arabinan);
~ 1.2 U/mg (on wheat arabinoxylan)

Unit Definition

One Unit of α-L-arabinofuranosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-L-arabinofuranoside (5 mM) in sodium acetate buffer (100 mM), pH 4.0 at 40°C.

Temperature Optima (°C)40
pH Optima4
Package Dimensions0.70 in x 0.70 in x 1.60 in
Package Weight0.03 lb