Megazyme® Sucrase (from yeast)
300 Units (on sucrose)
SKU No. 700004241 | Catalog No. E-SUCR
AOAC OMA
High purity Sucrase (from yeast) for use in research, biochemical enzyme assays and analytical testing applications. Sucrase (E-SUCR) and Fructanase (E-FRMXLQ or E-FRMXPD) are used in the measurement of fructan in foods in various methods.
| Specifications | ||
|---|---|---|
| Brand | Megazyme® | |
| Approvals | AOAC OMA 2016.06 | |
| EC Number | 3.2.1.20 | |
| Storage Conditions | Storage Temperature: Below -10°C Stability: > 1 year under recommended storage conditions. Check vial for details | |
| Application Examples | Applications for the removal of sucrose in various analytical procedures in the cereals, food and feeds, fermentation and beverage industries. | |
| Formulation | Supplied as a lyophilised powder | |
| Physical Form | Powder | |
| CAS Number | 9001-42-7 | |
| Synonyms | alpha-glucosidase; alpha-D-glucoside glucohydrolase | |
| Source | Yeast | |
| Molecular Weight | 62000 | |
| Expression | Purified from Yeast | |
| Specificity | Hydrolysis of terminal, non-reducing (1,4)-linked α-D-glucose residues with release of D-glucose. | |
| Specific Activity | ~ 20 U/mg (30°C, pH 6.8 on sucrose) | |
| Unit Definition | One Unit of sucrase activity is defined as the amount of enzyme required to release one μmole of glucose per minute from sucrose (10 mM) in sodium maleate buffer (100 mM), pH 6.8 at 30°C. | |
| Temperature Optima (°C) | 30 | |
| Package Dimensions | 0.80 in x 0.80 in x 1.90 in | |
| Package Weight | 0.03 lb | |
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