Megazyme® Fructanase Mixture (powder)
SKU No.  700004216  | Catalog No.  E-FRMXPD
Top

Megazyme® Fructanase Mixture (powder)

20,000 Units

SKU No.  700004216  |  Catalog No.  E-FRMXPD

AOAC OMA

Learn more
Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
To order or for more information contact our food safety team
To order or for more information contact our food safety team

High purity Fructanase Mixture (powder) for use in research, biochemical enzyme assays and analytical testing applications.

Components: exo-Inulinase 20,000 U (on kestose at 40°C), endo-inulinase 1000 U (on fructan at 40°C).

Fructanase, along with Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY), can also be utilised in the enzyme hydrolysis step for determining polydextrose a low molecular weight dietary fiber in food samples.

Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan and Polydextrose.

Megazyme_Scheme-E-FRMXPD.png
Specifications
BrandMegazyme®
Approvals

For use with AOAC OMA 997.08, 2000.11, 2016.06

EC Number

endo-Inulinase: 3.2.1.7,
exo-Inulinase: 3.2.1.80

Storage Conditions

Storage Temperature: Below -10°C

Stability: > 1 year under recommended storage conditions. Check vial for details

FormulationSupplied as a lyophilised powder
Physical FormPowder
CAS Number

9001-57-4,
9025-67-6,
37288-56-5

Synonyms

endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase

SourceAspergillus sp.
ExpressionFrom Aspergillus sp.
Specificity

endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.

Unit Definition

endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40°C.

Temperature Optima (°C)40
Package Dimensions0.80 in x 0.80 in x 1.90 in
Package Weight0.03 lb