Megazyme® Fructanase Mixture (liquid)
10 ml / 20,000 Units
SKU No. 700004215 | Catalog No. E-FRMXLQ
AOAC OMA
High purity Fructanase Mixture (liquid) for use in research, biochemical enzyme assays and analytical testing applications.
Components: exo-inulinase 2,000 U/ml (on kestose, at 40°C), endo-inulinase ˜ 100 U/ml (on fructan at 40°C), α-galactosidase < 0.08 U/ml (on polygalacturonic acid at 40°C), β-Glucanase < 0.04 U/ml (on β-glucan at 40°C) and pectinase < 0.07 U/ml (on pectin at 40°C).
Fructanase, along with Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY), can also be utilised in the enzyme hydrolysis step for determining polydextrose a low molecular weight dietary fiber in food samples.
Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan and polydextrose.
Fructanase and sucrase (E-SUCR) may also be utilized in the enzymatic hydrolysis of fructans during analytical procedures designed to quantify fructan content in food samples according to the relevant AOAC and GB standard methods.
| Specifications | ||
|---|---|---|
| Brand | Megazyme® | |
| Approvals |
For use with AOAC OMA 997.08, 2000.11, 2016.06 |
|
| EC Number |
endo-Inulinase: 3.2.1.7, |
|
| Storage Conditions |
Storage Temperature: Below -10°C Stability: > 1 year under recommended storage conditions. Check vial for details |
|
| Formulation | In 50% (v/v) glycerol | |
| Physical Form | Solution | |
| CAS Number |
9001-57-4, |
|
| Synonyms |
endo-inulinase: 1-beta-D-fructan fructanohydrolase |
|
| Source | Aspergillus sp. | |
| Expression | From Aspergillus sp. | |
| Specificity |
endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin. |
|
| Unit Definition |
endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5. |
|
| Temperature Optima (°C) | 40 | |
| Package Dimensions | 0.80 in x 0.80 in x 1.90 in | |
| Package Weight | 0.06 lb | |
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