Reveal® for Histamine is a single-step lateral flow assay based on a competitive immunoassay format intended for the visual screening of histamine in scrombroid species of fish, such as tuna and mahi-mahi.
Specifications
Analyte Histamine
Brand Reveal®
Cross Reactivity Specific for histamine. No cross-reactivity with other biogenic amines.
Limit Of Detection 50 ppm
Package Dimensions 8.30 IN x 5.10 IN x 2.50 IN
Package Weight 1.10 LB
Platform Lateral Flow
Quantity per Package 25 tests
Result Type Qualitative
Testing Time 5.00 Minutes

  1. 25 histamine test strips
  2. 25 sample cups
  3. 25 bottles of 7 mL sample diluent
  1. Graduated cylinder (Neogen® item 9368)
  2. Sample extraction bottles, 250 mL (Neogen item 9399)
  3. Sample collection tubes (Neogen item 9421)
  4. Blender (Neogen item 9493, 9477)
  5. Scale capable of weighing 10–50 grams (Neogen item 9427)
  6. Pipettor, 100 µL (Neogen items 9272, 9278, 9276)
  7. Pipette tips (Neogen items 9410, 9407, 9417)
  8. Paper towels or equivalent absorbent material
  9. Rack for Reveal sample cups (Neogen item 9475)
  10. Timer (Neogen item 9426)
  11. Waterproof marker
  12. Distilled or deionized water
  13. AccuScan reader system (Neogen item 9565) (Optional)

Reveal for Histamine is a single-step lateral flow assay based on a competitive immunoassay format. The extract is wicked through a reagent zone, which contains histamine-specific antibodies conjugated to coloured particles. If histamine is present, it will be captured by the particle-antibody complex. The histamine-antibody-particle complex then is wicked onto a membrane, which contains a zone of histamine conjugated to a protein carrier. This zone captures only uncomplexed antibody-particle conjugate, allowing the coloured particles to concentrate and form a visible line. The membrane also contains a control zone where the antibody-particle complex present in the reagent zone is captured by an antibody, forming a visible line. The control line will always form regardless of the presence of histamine, ensuring the strip is functioning properly. In addition, the control line also is used as a reference for comparison to the test zone for visual interpretation of the test results. If histamine is present above a pre-determined limit, a line lighter than the control line or no line will form. Therefore, a positive sample will result in a light line or absence of a line in the test zone. Alternatively, a negative sample will form a line of the same or darker intensity as the control line in the test zone.

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


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