NeoColumn™ for Ochratoxin

Item No.  8640

  • High performance immunoaffinity column
  • Unique leak-proof stopper with "wings" for easy removal
  • State of the art beaded gel bed for maximum consistency of performance
  • Provides excellent recoveries of ochratoxin on a variety of matrix
  • Performance exceeds current EU and CEN regulatory requirements
$865.50
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NeoColumn™ for Ochratoxin A is a high performance immunoaffinity column designed for clean-up and concetration of sample prior to running on an HPLC system or Veratox®. NeoColumn is suitable for use in the determination of ochratoxin A in a wide range of commodities such as cereals, dried fruits, beer and wine, and green and roast coffee.
Specifications
Analyte Ochratoxin
Brand NeoColumn™
Limit of Detection 0.1 ppb
Package Dimensions 10.30 IN x 5.30 IN x 3.80 IN
Package Weight 0.50 LB
Platform Immunoaffinity
Quantity per Package 50 columns
Result Type Quantitative

NeoColumn for Ochratoxin WB (Neogen® item 8640)
  1. Scale, electronic (digital) (Neogen item 9427)
  2. Glassware/plasticware
  3. Laboratory blender (Neogen item 9493)
  4. Centrifuge or filter paper/filter syringe (Neogen item 9429, 9420)
  5. Extraction solvents (HPLC grade)
  6. Distilled water (HPLC grade)
  7. Phosphate Buffered Saline (PBS) (Neogen item 8425)
  8. Acetic acid
  9. Clamp stand (Neogen item 9358)
  10. Column rack (Neogen item 9359)
  11. Pipettes and tips (Neogen items 9463, 9464 and 9487)
  12. Glass or resin – treated (Sigmacote or equivalent) plastic syringe barrel (10 mL and pump unit (Neogen item 9365)
  13. Syringe adapter (Neogen item 9371)
  14. pH meter
  15. Ochratoxin A standard (see page 5)
  16. Clarification compound for red wine (Neogen item 8652)
  17. Sodium hypochlorite solution/tablets
  18. Methanol (HPLC grade needed for elution.)
  19. Whatman no. 4 filters (Neogen item 9429)
  20. Whatman GF/A filters (Neogen item 9358)
  21. Acentonitrile (For HPLC testing only)
  22. Sodium bicarbonate (For HPLC testing only)
  23. Tween
  24. Tris
  25. Sodium hydroxide

Technology

An immunoaffinity column that contains antibodies specific to ochratoxin. Ochratoxin is extracted from a ground sample by blending and filtering. Extracted toxin in the filtrate is sampled and diluted with methanol. The diluted extract is filtered and applied to the column. Positive pressure is used to induce flow through the column allowing the antibody to capture any ochratoxin present. Then, the column is washed to remove any unbound materials. Bound ochratoxin is eluted using acetic acid/HPLC grade methanol (2:98) and is detected by HPLC or Veratox® enzyme-linked immunosorbent assay (ELISA) methods.

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Validation

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