The E. coli Vial (S2-EC) is used to detect Escherichia coli species. The S2-EC vial contains a broth enrichment medium with a fluorescent indicator. The vial has an assay time of 18 hours for most applications. The E. coli vial utilizes MUG, the most commonly used fluorogenic substrate to detect the glucuronidase activity of E. coli organisms. The detection system features an agar plug through which optical measurements are performed, monitoring the fluorescent change in the liquid medium.
Specifications
Analyte E. coli
Brand Soleris®
Incubation Time 14-24 Hours
Limit Of Detection Presence/Absence
Package Dimensions 11.90 IN x 11.00 IN x 2.80 IN
Package Weight 4.70 LB
Platform Rapid Microbiology
Quantity per Package 100 vials
Result Type Quantitative
Testing Time 14.00 - 24.00 Hours
Volume 9 mL

  1. Soleris® Automated System (BSX128, BSX32 or BLX-32)

S2-EC, E. coli Vial:

  1. Tryptic Soy Broth, 90 mL (BLX-TSB90
    1. If required, use a designated neutralization broth, such as D/E Neutralizer, TAT Broth, Modified Letheen Broth, etc.
  2. S2-ECM Soleris E. coli MUG Supplement

Dependent on sample tested:

  1. Sterile 1 N to 5 N sodium hydroxide (NaOH) and/or hydrochloric acid (HCl)
  2. pH meter or pH paper

E. coli confirmation

  1. Kovac’s Reagent (KO-122)

Additional Information

FOR USE WITH BIOLUMIX INSTRUMENT ONLY

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