The Reveal® 2.0 for Salmonella test provides for the rapid recovery of Salmonella in chicken carcass rinse, raw ground turkey, raw ground beef, hot dogs, raw shrimp, ready-to-eat meal products, dry pet food, ice cream, fresh spinach, cantaloupe, peanut butter, swabs from stainless steel surfaces, and sprout irrigation water samples allowing detection and presumptive identification of Salmonella within 24 hours.
Specifications
Analyte Salmonella
Approvals AOAC-RI 960801
Brand Reveal®
Limit Of Detection 1 cfu/analytical unit
Package Dimensions 8.50 IN x 5.50 IN x 1.25 IN
Package Weight 0.20 LB
Platform Lateral Flow
Quantity per Package 20 tests
Result Type Presence/Absence
Testing Time 15.00 Minutes
Time to Result 24 - 48 Hours

  1. 20 Reveal 2.0 for Salmonella test devices
  2. 20 disposable transfer pipettes
  3. 20 reaction cups
  1. Scale capable of weighing a minimum of 25 g (Neogen® item 9427)
  2. Timer (Neogen items 9452, 9426)
  3. Sample cup rack (Neogen item 9475)
  4. Sterile deionized or distilled water
  5. Incubator capable of maintaining 42 ± 1°C (Neogen item 9735)
  6. Incubator capable of maintaining 36 ± 1°C (Neogen item 9735)
  7. Graduated cup
  8. Stomacher-type bags

Test Systems:

NOTE: The Reveal 2.0 for Salmonella device requires the use of certain enrichment media and supplies. These can be purchased as part of a test system or individually.

  1. Reveal 2.0 one-step system (contains devices, 1x RV selective medium and sampling bags for 20 tests) (Neogen item 9802)
  2. Reveal 2.0 complete system with RV (contains devices, Revive, 2x RV selective medium and sampling bags for 20 tests) (Neogen item 9803)
  3. Reveal 2.0 environmental system (contains devices, Revive, 2x RV, sampling bags and environmental sample kits for 20 tests) (Neogen item 9804)
  4. Reveal 2.0 complete system with RV and M-broth (contains devices, Revive, 2x RV selective medium, M-broth and sampling bags for 20 tests) (Neogen item 9805)

Media:

NOTE: Media can be purchased individually or as part of a test system.

  1. 20 bottles of Revive medium (Neogen item 9705)
  2. 20 bottles 2x concentrated Rappaport-Vassiliadis (2x RV) (Neogen item 9715)
  3. 20 bottles 1x Rappaport-Vassiliadis (1x RV) (Neogen item 9729)
  4. 20 bottles M-Broth (Neogen item 9722)
  5. Lactose Broth (Neogen item 7141)

Optional Materials

  1. AccuScan® reader
  2. Stomacher 400 machine or equivalent

The system utilises Revive medium, which provides Salmonella with readily available nutrients and other factors required for its recovery from a stressed or injured condition. After a brief enrichment in Revive, selective enrichment in Rappaport-Vassiliadis (RV) then favours Salmonella growth to levels detectable by the Reveal test device.

For certain matrices, the initial nonselective enrichment step may be bypassed and the sample introduced directly into the RV medium.

Another enrichment option enables the Reveal test device to detect Salmonella spp. in matrices that are more difficult due to viscosity, pigment, or containing an extremely high level of nonviable cells. M-broth was selected for post enrichment as it is established in several standard procedures recommended in enzyme assays used in the food industry.

A portion (200 µL) of the enrichment culture is placed into the sample cup. The test device is placed into the sample and allowed to develop at ambient temperature for 15 minutes. The sample is wicked through a reagent zone that contains specific anti-Salmonella antibodies conjugated to colloidal gold particles. If antigens are present in the sample, they will bind to the gold-conjugated antibodies. This antigen-antibody complex then leaves the reagent zone and travels through the nitrocellulose membrane which contains a zone of anti-Salmonella antibodies. The immune complex with gold conjugate is captured and aggregates in this zone, thus displaying a visible line. The remainder of the sample continues to migrate to the end of the membrane where it is deposited into a waste reservoir.

The reagent zone also contains gold conjugate of a proprietary antigen, which is eluted by the sample regardless of the presence of Salmonella antigen. The gold-conjugated control indicator migrates through the membrane to the negative control capture zone (antibody to the proprietary antigen), where it is captured and aggregated to form a visible line. Regardless of the presence or absence of the Salmonella antigen, the control line will form in the control zone, ensuring the test is working properly.

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


Support

Validation

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