Modified Semi-Solid Rappaport-Vassiliadis (MSRV) Agar

Item No.  NCM0067A

$131.00
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Modified Semi-Solid Rappaport-Vassiliadis (MSRV) Agar is used with Novobiocin for the rapid detection of motile Salmonella spp. and is not intended for use in the diagnosis of disease or other conditions in humans. MSRV was developed in 1986 by De Smedt, Bolderdijk and Rappold as a rapid means of Salmonella detection. The medium, based upon Rappaport Vassiliadis broth, is inoculated directly from the preenrichment medium, in the center of the plate. Motile organisms spread from the center in the semi-solid agar, but non-salmonellas are inhibited by the selective agents. After overnight incubation the use of polyvalent salmonella antisera or a latex kit can confirm the presence of a Salmonella. Alternatively, a paper disc wetted with polyvalent H antiserum can be placed 1/3 of the way from the edge of the dish, and will signal the presence of a Salmonella by inhibiting the mobility of the organism around the disc. Using this medium De Smedt and Bolderdijk have reported the possibility of detecting Salmonella in 24hrs (1987).  
Specifications
Analyte Salmonella spp.
Brand NEOGEN Culture Media
Formula
Formula Liter
Tryptone 2.3 g/L
Meat Peptone 2.3 g/L
Acid Hydrolysed Casein 4.65 g/L
Sodium Chloride 7.34 g/L
Potassium Dihydrogen Phosphate 1.5 g/L
Magnesium Chloride 10.9 g/L
Malachite Green 0.037 g/L
Agar 2.5 g/L
Grams per Litre 31.50
Litres per 500g 16.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 870.00
Preparation
  1. Suspend 31.5 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. DO NOT AUTOCLAVE.
  4. Cool to 45-50°C and add 2 vials of X150 or 10mL of 7985 Novobiocin Supplement.
  5. Mix well and dispense into petri dishes.
Previous Item Number Product was previously known as Acumedia Product: Rappaport-Vassiliadis (MSRV) Medium Semisolid Modified; and LabM Product: LAB150 MSRV Medium (Semi-Solid Rappaport Medium)

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