Harlequin® Tryptone Bile Glucuronide Agar (TBX)

Item No.  NCM1001A

$268.00
$2,545.00
$4,981.00
$12,225.00
$30.50
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Harlequin® Tryptone Bile Glucuronide Agar (TBX) is used for the enumeration of E. coli in food and animal feed and is not intended for use in the diagnosis of disease or other conditions in humans. Tryptone Bile Glucuronide Agar is recommended by ISO 16649-1:2001, ISO 16649-2:2001 and ISO 16649-3:2015 for enumeration of β-glucuronidase positive Escherichia coli in products intended for human consumption or for the feeding of animals. The medium contains tryptone as a source of nitrogen and amino acids, bile salts act as a selective against Gram-positive organisms and agar is the gelling agent. 5-bromo-4-chloro-3-indolyl-β-D-glucuronide (X-glucuronide) is a chromogen for the detection of the β-D-glucuronidase enzyme which is present in most E. coli* but not other coliforms. The intracellular enzyme present in E. coli is able to break the bond between the substrate and the chromophore which dimerizes is the presence of oxygen to form an insoluble colored compound within the cell. The accumulation of the colored chromophore results in blue colonies on the agar. *96-97% of E.coli are β-D-glucuronidase positive. A notable exception is E. coli O157:H7  
Specifications
Analyte E. coli
Brand NEOGEN Culture Media
Formula
Formula Liter
Enzymatic Digest of Casein 20.0 g/L
Bile Salts No. 1.5 g/L
X-glucuronide 0.075 g/L
Agar 15.0 g/L
Grams per Litre 36.50
Litres per 500g 14.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 750.00
Preparation
  1. Suspend 36.5 grams of the medium in 1 liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.
Previous Item Number Product was previously known as Acumedia Product: 7692 Harlequin Tryptone Bile Glucuronide Agar (TBX); and LabM Product: HAL003 Harlequin™ TBGA/TBX (Tryptone Bile Glucuronide Agar)

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