Cronobacter Selective Broth

Item No.  NCM0227A

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Cronobacter Selective Broth (CSB) is recommended by ISO 22964 for the isolation and identification of Cronobacter spp. (formerly Enterobacter sakazakii), and is not intended for use in the diagnosis of disease or other conditions in humans. Although rarely causing infections in immunocompetent adults, members of the Cronobacter genus have been implicated in sepsis, meningitis and necrotizing enterocolitis with a high death rate in neonates. These opportunistic pathogens are common in the environment and their ability to survive desiccation presents a significant risk for post-pasteurization contamination and survival in spray dried milk products. Enzymatic digest of animal tissues and meat extract provide a source of nitrogen and essential vitamins. High levels of sodium chloride and sucrose, the addition of vancomycin and an elevated incubation temperature produce a selective and specific medium. Cronobacter spp. ferment the sucrose in this broth, resulting in a decrease in pH and thus a change of color from purple to yellow or red due to the presence of the pH indicator bromocresol purple. Most other organisms do not grow in this medium, while E. coli are suppressed, resulting in a turbid medium with no changes in color. According to ISO 22964:2017, subculture is performed after pre-enrichment in Buffered Peptone Water (BPW).
Analyte Cronobacter spp.
Brand NEOGEN Culture Media
Formula Liter
Enzymatic Digest of Animal Tissues 10.0 g/L
Meat Extract 3.0 g/L
Sodium Chloride 5.0 g/L
Bromocresol Purple 0.04 g/L
Sucrose 10.0 g/L
Grams per Litre 28.00
Litres per 500g 17.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 980.00
  1. Disperse 28 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium if necessary.
  3. Autoclave at 121 °C for 15 minutes.
  4. Cool to ambient temperature, then add 2 vials of NCM4004-0.5* Vancomycin (10mg/L) supplement, each reconstituted using 5mL of sterile deionized/RO water.

*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.

Previous Item Number Product was previously known as LabM Product: LAB226 Cronobacter Selective Broth (ISO)



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