BCYE Agar (Legionella Isolation Medium)

Item No.  NCM0037B

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BCYE Agar is used (with appropriate supplementation) for the isolation of Legionella spp. from water and environmental samples. BCYE Agar is not intended for use in the diagnosis of disease or other conditions in humans. In 1977, McDade et al. identified Legionella pneumophila as the causative agent of Legionnaires’ disease, a multisystem disease manifested primarily by pneumonia. In 1978 a new medium, F-G Agar, resulted in improved growth of L. pneumophila, a very fastidious organism. Freely et al. modified F-C Agar by substituting yeast extract as a vitamin source and replacing starch with activated charcoal, producing Charcoal Yeast Extract (CYE) Agar. In 1980, Pasculle et al. reported that CYE Agar could be improved by the addition of ACES (N-2-acetamido-2-aminoethane sulfonic acid) buffer. One year later, Edelstein further increased the sensitivity of the medium by adding the potassium salt of alpha-ketoglutaric acid.
Specifications
Analyte Legionella spp.
Brand NEOGEN Culture Media
Formula
Formula Liter
Yeast Extract 10.0 g/L
Charcoal 2.0 g/L
Ferric Pyrophosphate 0.25 g/L
ACES Buffer 10.0 g/L
Potassium Carbonate 2.3 g/L
Agar 14.0 g/L
Grams per Litre 38.50
Litres per 500g 13.00
Mass 5 kg
Package Dimensions 11.50 IN x 11.50 IN x 10.50 IN
Package Weight 14.00 LB
Plates per 500g 710.00
Preparation
  1. Suspend 38.5 g of the medium in 1L of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 110°C for 10 minutes. Cool to 45 - 50°C.
  4. Aseptically add 2 vials of X196 BCYE growth supplement to make BCYE agar with Legionella growth supplements.
  5. To make GVPC selective medium additionally add 2 vials of X195 GVPC selective supplement
  6. Mix thoroughly and continuing mixing throughout dispensing. NOTE: X196 supplement can be substituted with X197 supplement (without the addition of X195 selective supplement) to prepare presumptive identification plates.
Previous Item Number Product was previously known as Acumedia Product: 7728 BCYE Agar Base; and LabM Product: LAB195 BCYE Legionella Isolation Medium

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