ANSR® for Salmonella

Item No.  9870

  • Total time to results 10 – 26 hours (25 g or 325 g)
  • Irradiated media for easy preparations/single-step enrichments
  • AOAC OMA approved as a confirmatory procedure
$1,176.00
In stock
Out of stock
Expect availability 12/28/2020
Quantity:
Minimum quantity required: 0
ANSR® for Salmonella provides rapid recovery and detection of Salmonella in food or environmental samples in 10 – 26 hours.
Specifications
Analyte Salmonella
Approvals
  • AOAC 061203
  • AFNOR NF Validation EN ISO 16140
Brand ANSR®
Incubation Time 10-26 Hours
Limit Of Detection 1 cfu/analytical unit
Package Dimensions 7.80 IN x 5.20 IN x 4.10 IN
Package Weight 0.80 LB
Platform Molecular
Quantity per Package 96 tests
Result Type Qualitative
Testing Time 10.00 Minutes

  1. 12 strips of 8 cluster tubes in a rack, 1.2 mL
  2. 12 strips of 8 reaction tubes, 200 µL, containing lyophilized ANSR for Salmonella reagents in 2 sealed foil pouches with desiccant pack
  3. 12 strips of 8 permanent caps for reaction tubes
  4. 1 bottle of lysis reagent suspension buffer, 60 mL
  5. 3 vials containing lyophilized lysis reagents
  6. 1 kit insert

Equipment Required

  1. ANSR reader (Neogen item 9828)
  2. Computer and software for connection to ANSR reader (Neogen item 9832)
  3. 1 dual heater block with lysis block inserts for 1.2 mL cluster tubes, 80 ± 2°C and 37 ± 2°C (Neogen items 9386-DUAL48, 9829-48)
  4. Pipettor, 100 – 1000 µL (Neogen item 9463)
  5. Pipette tip rack, 100 – 1000 µL, sterile, 96 tips (Neogen item 9487)
  6. Pipettor, 10 – 100 µL, 8-channel (Neogen item 9388)
  7. Pipette tips, 100 µL, sterile, filtered, 96 tips (Neogen item 9389)
  8. Vortex mixer, adjustable speed (Neogen item 9494)
  9. Stomacher (optional)
  10. 3 thermometers, traceable (Neogen item 9518)
  11. Timer, 3-channel (Neogen item 9426)
  12. Optional heater block with block insert for 0.2 mL reaction tubes, 56 ± 1°C (Neogen items 9386-48D, 9829-64)
  13. Webcam (Neogen item WEBCAM)
  14. ANSR ethernet cable (Neogen item 9835)
  15. Pipette pump, 10 mL (Neogen item 9277)
  16. Pippettes, sterile serological, 10 mL (Neogen item 8686)
  17. 40-slot, 20 mm test tube rack, autoclavable (Neogen item 9553)
  18. Pipettor, 20-200 µL (Neogen item 9276)

Other Materials Required

  1. Stomacher-type bags for sample enrichment. Filtered bags are recommended (Neogen item 6827 or equivalent)
  2. Graduated cylinder, 250 mL (Neogen item 9368 or equivalent)

Media Enrichment Broth Required

  1. Novobiocin, sodium salt (Neogen item 7985 or equivalent)
  2. Rappaport-Vassiliadis Broth (Neogen item 7512 or equivalent). For raw meat sample confirmation only.
  3. Phosphate Buffered Saline (PBS) (Neogen item 8425 or equivalent)
  4. Potassium iodide (Sigma no. 221945 or equivalent)
  5. Iodine (Sigma no. 207772 or equivalent)

Enrichment Broth Required (Dependent on sample type)

  1. ANSR for Salmonella Enrichment Broth 1 (Neogen item 9811) - use with processed meats and other processed foods with a moderate to high microbial loads such as hot dogs, raw almonds, peanut butter, black pepper and cocoa powder.
  2. ANSR for Salmonella Enrichment Broth 2 (Neogen item 9812) - use with raw meat, poultry carcass rinses, raw frozen shrimp, raw leafy greens and flours including soy.
  3. ANSR for Salmonella Enrichment Broth 3 (Neogen item 9813) - use with environmental (except poultry house samples) and low microbial loads such as oat cereal and ice cream.
  4. ANSR for Salmonella Enrichment Broth 3B (Neogen item 9817) - use with dry pet food and pasteurized egg products.
  5. Tetrathionate Broth Base, Hajna (TT Hajna) (Neogen item 7740A) - use with poultry house environmental samples.

Technology

  1. A special lysis reagent is added to the enrichment sample. Target pathogen DNA or RNA is released through the lysis step.
  2. The lysed sample is added to ANSR reagents. A special primer targets specific regions of the pathogen DNA and starts amplification of the DNA, creating millions of copies in minutes.
  3. The amplified segments of the pathogen DNA bind to special molecular beacons which then fluoresce and are detected by the ANSR reader.
  4. Results will be reported in 10 minutes and displayed as positive, negative or invalid.

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


Support

Validation

Our experts are here to help. If you need information beyond what is provided in Validation Reports under the Documents tab, please contact us and we will be happy to assist you. Learn More