Letheen Agar Base, Modified - 7495

Item No.  7495A

This item has been replaced by item NCM0130.
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Letheen Agar Base, Modified is used with Tween 80 (Polysorbate 80) for the isolation of microorganisms from cosmetics in a laboratory setting. Letheen Agar Base, Modified is not intended for use in the diagnosis of disease or other conditions in humans. In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Tween 80 (Polysorbate 80) to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds in testing of germicidal activity. Total neutralization of disinfectants is critical. Disinfectant residues can result in a false negative (no-growth) test. Letheen Agar Base, Modified is based on the formula described in FDA Bacteriological Analytical Manual, and a modification of Letheen Agar Base. Letheen Agar Base, Modified is recommended by the FDA for use in the microbiological testing of cosmetics.  
Specifications
Brand NEOGEN Culture Media
Formula
Formula Liter
Letheen Agar Base 25 g
Enzymatic Digest of Casein 10 g
Enzymatic Digest of Animal Tissue 10 g
Yeast Extract 2 g
Sodium Chloride 5 g
Sodium Bisulfite 0.1 g
Mass 500 g
Package Weight 1.20 LB
Preparation
  1. Suspend 52.1 g of the medium and 7 mL of Tween 80 (Polysorbate 80) in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

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