Veratox® for Gliadin

Item No.  8480

  • Accurate and easy-to-use
  • Cost effective microwell format
  • High specificity
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Veratox® for Gliadin/Gluten is used for the quantitative analysis of prolamins (wheat gliadin, rye secalin, and barley hordein) in ingredients, clean-in-place rinses and finished food products intended to be prolamins (gluten) free.
Analyte Gliadin/Gluten
Brand Veratox®
Limit of Detection 2.5 ppm gliadin
Package Dimensions 10.50 IN x 8.00 IN x 2.80 IN
Package Weight 0.80 LB
Platform ELISA
Prop 65 Warning
WARNING: This product can expose you to chemicals including thimerosal, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to
Quantity per Package 36 tests
Range of Quantitation 5.00 ppm gliadin - 50.00 ppm gliadin
Result Type Quantitative
Testing Time 30.00 Minutes

  1. 48 antibody-coated microwells
  2. 48 red-marked transfer wells
  3. 5 yellow-labeled bottles of 0, 5, 10, 20, and 50 ppm gliadin (0, 10, 20, 40 and 100 ppm gluten) controls (0, 12.5, 25, 50, and 125 ng/mL gliadin)
  4. 2 blue-labeled bottles of enzyme-labeled antibody conjugate
  5. 1 green-labeled bottle of K-Blue® Substrate
  6. 1 red-labeled bottle of Red Stop solution
  7. 40 mL of 10 mM PBS-Tween washing reagent in a wide mouth bottle. Each bottle can prepare 1 L in distilled or deionized water (pH 7.4)
  8. 1 foil pouch of 10 mM PBS dry powder containing enough powder to prepare 1 L of dilution solvent
  9. 50 g of extraction additive in 2 specimen cups
  10. Plastic scoop to measure extraction additive

Universal materials for all extraction and test methods

  1. Microwell Reader with 650 nm filter (Neogen® item 9303)
  2. Graduated cylinder 10-20 mL (Neogen item 9447)
  3. Graduated cylinder 250 mL (Neogen item 9368)
  4. 1-Liter bottle (Neogen item 9472)
  5. Pipettor, 50-200 µL adjustable (Neogen item 9276)
  6. Pipettor, 12-channel 50-200µL (Neogen item 9273)
  7. Pipette tips (Neogen item 9410, 9407, 9417)
  8. Wash bottle (Neogen item 9400)
  9. Well holder (Neogen item 9402)
  10. Timer (Neogen item 9426)
  11. Reagent boats, set of 3 (Neogen item 9435)
  12. 7 mL tube or vial (Neogen item 9468)
  13. Pipette pump for serological tubes (Neogen item 9277)
  14. Pipettes, sterile serological, 10 mL (pack of 100) (Neogen item 9415)
  15. Laboratory grade ethanol (≥190 proof)
  16. Distilled water
  17. Waterproof marker
  18. Paper towel or equivalent
  19. Special extraction additive for dark chocolate, cocoa and tannin (Neogen item 8482)
  20. Centrifuge (optional)
  21. Vortex (optional)

Non-heat treated method (extraction A or B)

  1. Tube, 50 mL (Neogen item 9381) or bottle, 125 mL (Neogen item 9398)
  2. Scale ± 0.1 g (Neogen item 9427, 9395)
  3. Orbital rotator (Neogen item 9396) or shaker to hold 50 mL tube

Heat-treated cocktail method (extraction C)

  1. Gliadin renaturing cocktail solution for heat processed samples (Neogen item 8515, 8515B, 8515S)
  2. Orbital rotator (Neogen item 9396)
  3. Scale, ± 0.01g (Neogen Item 9395)
  4. Water bath or oven at 50°C


Veratox for Gliadin is a sandwich enzyme-linked immunosorbent assay (S-ELISA). Gliadin is extracted from samples with a 40% ethanol solution by shaking in a shaker or rotator. Extract is diluted in Phosphate Buffered Saline (PBS) and diluted samples are added to antibody-coated wells (capture antibody) where gliadin will bind to the antibody during an incubation period. Any unbound gliadin is washed away and a second antibody, which is enzyme labelled (detector antibody) is added. The detector antibody binds to the gliadin during another incubation period. Unbound enzyme-labelled antibody is washed away and a one step substrate is added. Colour develops as a result of the presence of bound-labelled antibody. A stopping reagent is added and the colour of the solution is observed. Blue colour indicates samples containing high levels of gliadin while purple or red samples contain little or no gliadin. The optical densities of the controls form a standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of gliadin in parts per million (ppm).


Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.



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