Veratox® for Gliadin
Item No. 8480
- Accurate and easy-to-use
- Cost effective microwell format
- High specificity
Specifications | |
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Analyte | Gliadin/Gluten |
Brand | Veratox® |
Limit of Detection | 2.5 ppm gliadin |
Package Dimensions | 10.50 IN x 8.00 IN x 2.80 IN |
Package Weight | 0.80 LB |
Platform | ELISA |
Prop 65 Warning |
![]() WARNING: This product can expose you to chemicals including thimerosal, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov.
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Quantity per Package | 36 tests |
Range of Quantitation | 5.00 ppm gliadin - 50.00 ppm gliadin |
Result Type | Quantitative |
Testing Time | 30.00 Minutes |
Documents
Technology
Veratox for Gliadin is a sandwich enzyme-linked immunosorbent assay (S-ELISA). Gliadin is extracted from samples with a 40% ethanol solution by shaking in a shaker or rotator. Extract is diluted in Phosphate Buffered Saline (PBS) and diluted samples are added to antibody-coated wells (capture antibody) where gliadin will bind to the antibody during an incubation period. Any unbound gliadin is washed away and a second antibody, which is enzyme labelled (detector antibody) is added. The detector antibody binds to the gliadin during another incubation period. Unbound enzyme-labelled antibody is washed away and a one step substrate is added. Colour develops as a result of the presence of bound-labelled antibody. A stopping reagent is added and the colour of the solution is observed. Blue colour indicates samples containing high levels of gliadin while purple or red samples contain little or no gliadin. The optical densities of the controls form a standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of gliadin in parts per million (ppm).
Training
Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.