Veratox® for Gliadin R5

Item No.  8510

  • Accurate and easy-to-use
  • Quantitate or screen samples
  • High specificity
$640.00
In stock
Out of stock
Expect availability in 2-4 weeks
Veratox® for Gliadin R5/Gluten is used for the quantitative analysis of ingredients, clean-in-place rinses and finished food products intended to be gluten free for the presence of gliadin and prolamins found in wheat, barley and rye.
Specifications
Analyte Gliadin/Gluten
Approvals AOAC-RI 061201
Brand Veratox®
Limit Of Detection 2.5 ppm gliadin
Package Dimensions 10.50 IN x 8.00 IN x 3.00 IN
Package Weight 0.90 LB
Platform ELISA
Prop 65 Warning
Warning
WARNING: This product can expose you to chemicals including thimerosal, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package 36 tests
Range of Quantitation 2.50 ppm gliadin - 40.00 ppm gliadin
Result Type Quantitative
Testing Time 30.00 Minutes

  1. 48 antibody-coated microwells
  2. 48 red-marked transfer wells
  3. 6 yellow-labeled bottles of 0, 2.5, 5, 10, 20, and 40 ppm gliadin controls (0, 5, 10, 20, 40, and 80 ng/mL gliadin)
  4. 2 blue-labeled bottles of enzyme-labeled antibody conjugate
  5. 1 green-labeled bottle of K-Blue® Substrate
  6. 1 red-labeled bottle of Red Stop solution
  7. 40 mL of 10 mM PBS-Tween washing reagent in a widemouth bottle. Each bottle can prepare 1 L in distilled or deionized water (pH 7.4)
  8. 1 foil pouch sample diluent concentrate of 10 mM PBS dry powder containing enough powder to prepare 1 L of dilution buffer
  9. 50 g of extraction additive in 2 specimen cups
  10. Plastic scoop to measure extraction additive
  1. Microwell Reader with 650 nm filter (Neogen® item 9303)
  2. Graduated cylinder 10–20 mL (Neogen item 9447)
  3. Graduated cylinder 250 mL (Neogen item 9368)
  4. 1–Liter bottle (Neogen item 9472)
  5. Pipettor, 50-200 µL adjustable (Neogen item 9276)
  6. Pipettor, 12-channel 50-200 µL ((Neogen item 9273)
  7. Pipette tips (Neogen item 9410, 9407, 9417)
  8. Wash bottle (Neogen item 9400)
  9. Well holder (Neogen item 9402)
  10. Timer (Neogen item 9426)
  11. Reagent boats, set of 3 (Neogen item 9435)
  12. 7 mL tube or vial (Neogen item 9468)
  13. Pipette pump for serological tubes (Neogen item 9277)
  14. Pipettes, sterile serological, 10 mL (pk of 100) (Neogen item 9415)
  15. Laboratory grade ethanol (190 proof)
  16. Distilled water
  17. Waterproof marker
  18. Paper towel or equivalent
  19. Centrifuge (optional)
  20. Vortex (optional)

Non-heat treated method (extraction A or B)

  1. Tube, 50 mL (Neogen item 9381) or bottle, 125 mL (Neogen item 9398)
  2. Scale ± 0.1 g (Neogen item 9427, 9395)
  3. Orbital rotator (Neogen item 9396) or shaker to hold 50 mL tube

Heat-treated cocktail method (extraction C)

  1. Gliadin renaturing cocktail solution for heat processed samples (Neogen item 8515, 8515B, 8515S)
  2. Orbital rotator (Neogen item 9396)
  3. Scale, ± 0.01g (Neogen Item 9395)
  4. Water bath or oven at 50°C

Veratox for Gliadin R5 is a sandwich enzyme-linked immunosorbent assay (S-ELISA). Gliadin is extracted from samples with a 60% ethanol solution by shaking in a shaker or rotator. The extract is diluted in Phosphate Buffered Saline (PBS) and diluted samples are added to R5 antibody-coated wells (capture antibody) where gliadin will bind to the antibody during an incubation period. Any unbound gliadin is washed away and a second antibody, which is R5 enzyme-labelled (detector antibody) is added. The detector antibody binds to the gliadin during another incubation period. Unbound enzyme-labelled antibody is washed away and a one stop substrate is added. Colour develops as a result of the presence of bound-labelled antibody. A stopping reagent is added and the colour of the solution is observed. Blue indicates samples containing high levels of gliadin while purple or red samples contain little or no gliadin. The optical densities of the controls form a standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of gliadin in parts per million (ppm).

Training

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