Veratox® for Coconut Allergen is intended for the full quantitative analysis or simple screening of coconut protein residues on surfaces, clean-inplace rinses and in food products such as beverages, cookies, crackers, chocolate bars, ice cream and cereals. The test provides food processors a method to easily determine if an ingredient or product has been subjected to cross-contact with coconut residue.
Specifications
Analyte Coconut, Tree Nuts
Brand Veratox®
Limit Of Detection 1 ppm coconut
Package Dimensions 6.40 IN x 4.20 IN x 0.20 IN
Package Weight 0.90 LB
Platform ELISA
Prop 65 Warning
Warning
WARNING: This product can expose you to chemicals including thimerosal, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package 38 tests
Range of Quantitation 1.00 ppm coconut - 25.00 ppm coconut
Testing Time 30.00 Minutes

48 antibody-coated microwells 48 red-marked transfer wells 6 yellow-labeled bottles of 0, 1, 2.5, 5, 10, and 25 ppm coconut controls 2 blue-labeled bottles of enzyme-labeled antibody conjugate 1 green-labeled bottle of K-Blue® Substrate 1 red-labeled bottle of Red Stop solution 5 foil pouches of phosphate-buffered saline (PBS) powder. Each pouch contains PBS powder sufficient to prepare 1.0 L of 10 mM PBS buffer at pH 7.4. 40 mL of 10 mM PBS-Tween washing reagent concentrate in a wide mouth bottle. Each bottle contains wash reagent concentrate sufficient to prepare 1 L in distilled or deionized water (pH 7.4) 50 g of extraction additive in a specimen cup Plastic scoop to measure extraction additive
Allergen Extraction Kit (Neogen item 8429) 20 disposable plastic extraction bottles 20 sample collection tubes (12 x 75 mm) with caps Shaker water bath capable of maintaining 60 ± 1°C with holders for 250 mL disposable plastic bottles (Neogen items 9298, 9299) Whatman No. 4 filters or equivalent (Neogen item 9429) Centrifuge (optional) Pipettor, 50–200 µL, adjustable (Neogen item 9276) Pipettor, 12-channel (Neogen item 9273) Pipette tips (Neogen item 9410, 9407, 9417) Timer (Neogen items 9426, 9452) Microwell reader with a 650 nm filter (Neogen item 9303) 1 L bottle to prepare washing solution (Neogen item 9472) 1 L heat safe bottle to prepare extract solution (Neogen item 9472) Paper towels or equivalent absorbent material Microwell holder (Neogen item 9402) Waterproof marker Wash bottle (Neogen item 9400) Distilled or deionized water 3 reagent boats for 12-channel pipettor (Neogen item 9435) Graduated cylinder capable of measuring 125 mL (Neogen item 9368) Scale capable of weighing 5 ± 0.1 g (Neogen item 9427)

The Veratox for Coconut Allergen test is a sandwich enzyme-linked immunosorbent assay (S-ELISA). Coconut protein is extracted from samples with a phosphate buffered salt solution (PBS) by shaking in a heated water bath and clarified by centrifugation or filtration. The extracted coconut protein is added to antibody-coated wells (capture antibody) where it binds to the antibody during an incubation. Next, a washing step is performed to remove unbound proteins, and then an enzyme- labelled antibody (detector antibody), is added to the microwells. The detector antibody binds coconut protein captured in the microwells. After a second washing step, the enzyme substrate is added. A blue colour will develop when the coconut protein is present as a result of the bound detector antibody. Lastly, Red Stop reagent is added to stop the colour development reaction. The optical density of colour development is measured in a microwell reader. The coconut protein concentration for unknown samples is determined by plotting the samples’ optical densities against those generated from a standard curve.

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


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