Alert® for Peanut Allergen is used as a screening tool to test for peanut residue in food products such as cookies, crackers, chocolate bars, ice cream and cereals, and on environmental surfaces.
Specifications
Analyte Peanut
Limit Of Detection 5 ppm
Package Dimensions 10.00 IN x 8.00 IN x 2.60 IN
Package Weight 0.80 LB
Platform ELISA
Prop 65 Warning
Warning
WARNING: This product can expose you to chemicals including thimerosal, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package 20 tests
Result Type Qualitative
Testing Time 30.00 Minutes

  1. 24 antibody-coated microwells
  2. 1 yellow-labeled dropper bottle of 5 ppm peanut control
  3. 1 blue-labeled dropper bottle of enzyme-labeled antibody conjugate
  4. 1 green-labeled dropper bottle of K-Blue® Substrate solution
  5. 1 red-labeled dropper bottle of Red Stop solution
  6. 20 unlabeled empty sample dropper bottles with tips
  7. 3 foil pouches of 10 mM PBS dry powder extraction solvent; each pouch contains enough powder to prepare 1 L of extraction solution
  8. 1 wide-mouth bottle of 10 mM PBS-Tween wash buffer concentrate; a bottle contains enough concentrate to prepare 1 L of wash buffer
  9. 2 – 50 g cups of extraction additive
  10. Plastic scoop to measure extraction additive
  1. Allergen Extraction Kit (Neogen® item 8429)
    1. 20 disposable extraction bottles
    2. 20 disposable transfer pipettes
  2. Allergen Environmental Swabbing Kit (Neogen item 8432S)
    1. 100 sterile swabs
    2. 100 disposable dropper tips
  3. High-speed blender with a 250 mL jar or 250 mL disposable plastic bottles (Neogen item #9493, #9477)
  4. Scale capable of weighing 5 ± 0.1 g (Neogen item 9427)
  5. Shaker water bath, hot plate or equivalent heat source capable of maintaining 60 ± 1°C
  6. Thermometer
  7. Whatman #4 filter paper or equivalent (Neogen item #9429)
  8. Two 1 L bottles to prepare washing and extraction solutions (Neogen item 9472)
  9. Paper towels or equivalent absorbent material
  10. Microwell holder (Neogen item 9402)
  11. Waterproof marker
  12. Wash bottle (Neogen item 9400)
  13. Distilled or deionized water
  14. Timer (Neogen item 9426)
  15. Graduated cylinder capable of measuring 125 mL (Neogen item 9368)

First, samples and controls are added to their respective wells. During an incubation, the target substance binds to the antibodies. The wells are washed to remove all unbound materials.

An enzyme conjugate (antibody chemically linked with an enzyme) is added to all wells. During an incubation, conjugate binds to the already bound target substance, forming a “sandwich.” The more target substance in the wells, the more conjugate that binds in the wells.

The wells are washed again to remove all unbound conjugate.

A substrate, which changes colour in the presence of the conjugate, is added to the wells. During an incubation, colour develops in proportion to the amount of conjugate in the wells. The more conjugate, the more colour, and the more target substance that is detected.

Results are read visually in a screening format—the more blue colour, the more target substance detected.

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Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


Support

Validation

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