Phenol Red Broth Base - 7148

Item No.  7148A

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Phenol Red Broth Base is used with carbohydrates for the differentiation of microorganisms on the basis of carbohydrate fermentation reactions in a laboratory setting. Phenol Red Broth Base is not intended for use in the diagnosis of disease or other conditions in humans. The fermentative properties of bacteria are valuable criteria in their identification. A basal medium for determining the fermentation reactions of microorganisms must be capable of supporting the growth of test organisms and free from fermentable carbohydrates. Vera used a fermentation test medium employing the pH indicator phenol red and obtained accurate results. Phenol Red Broth Base is recommended for use to determine the ability of organisms to ferment various carbohydrates. Various fermentable substances may be added in any desired concentration. The concentration of carbohydrate generally employed for testing fermentation reactions of bacteria is 0.5 to 1%. Some investigators prefer to use 1% rather than 0.5% to ensure against reversion of the reaction due to depletion of the carbohydrate.  
Brand NEOGEN Culture Media
Formula Liter
Enzymatic Digest of Casein 10 g
Sodium Chloride 5 g
Phenol Red 0.018 g
Mass 500 g
Package Weight 0.50 LB
  1. Suspend 15 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. If desired, add carbohydrates (5 – 10 grams). Autoclave at 121°C for 15 minutes.
  4. Alternatively, filtered sterilized carbohydrate solutions may be added to the cooled sterilized broth.




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