Oxford Listeria Agar Base - 7428

Item No.  7428A

This item has been replaced by item NCM0056.
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Oxford Listeria Agar Base is used with antimicrobics for the selective isolation of Listeria spp. in a laboratory setting. Oxford Listeria Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem food industries. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission included turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage, and river water. Oxford Listeria Agar Base is prepared according to the formulation of Curtis et al. Listeria spp. grow over a pH range of 5.0 – 9.6, and survive in food products with pH levels outside these parameters.  
Specifications
Brand NEOGEN Culture Media
Formula
Formula Liter
Columbia Blood Agar Base 39 g
Esculin 1 g
Ferric Ammonium Citrate 0.5 g
Lithium Chloride 15 g
Agar 2 g
Mass 500 g
Package Weight 0.50 LB
Preparation
  1. Suspend 57.5 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 10 – 15 minutes. Cool to 45 – 50°C.

Aseptically add a filtered sterilized aqueous solution of 10 mg colistin sulfate and 20 mg moxalactam.


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